Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles.

Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Research Abstract Details 

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Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Abstract Text:

S Ohkuma,H Noguchi,F Amano,D Mizuno,T Yasuda,

Apoferritin particles were found in mouse peritoneal macrophages cultured in vitro. They were found as 20S particles in the "ribosomal fraction" of macrophages labeled with L-[14C]glutamic acid. Possibilities that they were breakdown products of ribosomes or of other well-known contaminants of the ribosomal fraction were excluded because they did not incorporate [5-3H]uridine. They were resistant to RNase and were relatively resistant to detergent. The antibody against horse spleen apoferritin precipitated about 70% of the particles in the 20S region, judging by measurement of radioactivity. On in vitro incubation with Fe2+ and suitable oxidizing agents the sedimentation coefficient of 80% of the 20S particles changed to about 60S, which corresponds to that of ferritin. SDS-polyacrylamide gel electrophoresis revealed the presence of subunit structures with the same molecular size as that of mouse liver apoferritin. Under the electron microscope, the particles appeared spherical with a relatively uniform diameter of about 130 A.

Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Publishing Authors By Initials

S Ohkuma,H Noguchi,F Amano,D Mizuno,T Yasuda,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleosides: uridine research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleosides: uridine research

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Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 79

Page Numbers: 1365-76

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1976

Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Keywords Mesh Terms:

KEYWORDS: Uridine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles. Information

Substance Name: Apoferritins

Registry Number: 9013-31-4

Grant and Affiliation Information for Synthesis of apoferritin in mouse peritoneal macrophages. Characterization of 20 S particles.

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Country: JAPAN

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MEDLINETA: J Biochem

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