Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b.

Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Research Abstract Details 

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Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Abstract Text:

C Eguchi,K Suzuki,K Imahori,

In order to obtain a better understanding of the allosteric site of rabbit muscle phosphorylase b, nine AMP analogs having a bulky hydrophobic benzene ring were synthesized and tested for activity as activators or inhibitors. N6-Benzyl-AMP derivatives activated phosphorylase b to the same extent as AMP but bound to the enzyme more tightly than AMP. N6-p-nitrobenzyl-AMP had the highest affinity (Ka = 7.7 X 10(-7) M) for the AMP site. In an attempt to irreversibly modify the allosteric site of phosphorylase b, N6-p-bromoacetaminobenzyl-AMP was synthesized. Phosphorylase b was maximally activated upon incorporation of 1.0 mol of N6-p-bromoacetaminobenzyl-AMP per enzyme subunit, and its activity was approximately 90% of that of native phosphorylase b measured in the presence of AMP. The modified enzyme showed characteristics (e.g., kinetic parameters, stability, solubility, inhibition by glucose-6-phosphate, and state of aggregation) quite similar to those observed for the native enzyme in the presence of AMP. These results indicate that the AMP site of phosphorylase was specifically labeled by N6-p-bromoacetaminobenzyl-AMP. The nature of the allosteric site of phosphorylase b is discussed based on the results obtained.

Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Publishing Authors By Initials

C Eguchi,K Suzuki,K Imahori,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research

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Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 1401-11

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1977

Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Keywords Mesh Terms:

KEYWORDS: Structure-Activity Relationship

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b. Information

Substance Name: Phosphorylases

Registry Number: EC 2.4.1.-

Grant and Affiliation Information for Synthesis of AMP analogs and their use for studies on the allosteric site of rabbit muscle glycogen phosphorylase b.

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Country: JAPAN

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MEDLINETA: J Biochem

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