Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent.

Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Research Abstract Details 

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Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Abstract Text:

K Nagata,K Suzuki,K Imahori,

A reactive ATP analog, N6-(6-bromoacetamidohexyl)-AMP.PCP, was synthesized in an attempt to covalently label the binding sites for adenine nucleotides, especially ATP, of various enzymes which utilize adenine nucleotides as substrates, cofactors, inhibitors or allosteric effectors. This reagent rapidly inactivated rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (GPD), myokinase (MK), and creatine kinase (CK) under very mild conditions. Adenine nucleotide substrates prevented the inactivation. In the case of GPD, complete inactivation was observed when 1 mol of the reagent per mol of enzyme subunit was incorporated into the enzyme. These results indicate that the present ATP analog may be useful as an affinity labeling reagent for various adenine nucleotide-dependent enzymes.

Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Publishing Authors By Initials

K Nagata,K Suzuki,K Imahori,

For similar animals: chordata: vertebrates: mammals: lagomorpha: rabbits research abstracts see: animals: chordata: vertebrates: mammals: lagomorpha: rabbits research

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Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 83

Page Numbers: 599-606

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1978

Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Keywords Mesh Terms:

KEYWORDS: Rabbits

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synthesis of a reactive ATP analog and its preliminary application as an affinity labeling reagent. Information

Substance Name: Adenylate Kinase

Registry Number: EC 2.7.4.3

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Country: JAPAN

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MEDLINETA: J Biochem

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