Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems.

Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Research Abstract Details 

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Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Abstract Text:

Y Koresawa,S Miyagawa,M Ikawa,K Matsunami,M Yamada,R Shirakura,M Okabe,

The origin of the Cre recombinase gene is bacteriophage P1, and thus the codon usages are different from in mammals. In order to adapt this codon usage for mammals, we synthesized a "mammalian Cre recombinase gene" and examined its expression in Chinese hamster ovarian tumor (CHO) cells. Significant increases in protein production as well as mRNA levels were observed. When the recombination efficiency was compared using CHO cell transfectants having a cDNA containing loxP sites, the "mammalian Cre recombinase gene" recombined the loxP sites much more efficiently than the wild-type Cre recombinase gene.

Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Publishing Authors By Initials

Y Koresawa,S Miyagawa,M Ikawa,K Matsunami,M Yamada,R Shirakura,M Okabe,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 127

Page Numbers: 367-72

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 2000

Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems.

AFFILIATION: Division of Organ Transplantation, Biomedical Research Center, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka 565-0871, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

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