Synthesis and structural characterization of silk-like materials incorporated with an elastic motif.

Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Research Abstract Details 

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Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Abstract Text:

Juming Yao,Tetsuo Asakura,

Genetic engineering strategies were applied to synthesize silk-like materials, [(GVPGV)(2)GG(GAGAGS)(3)AS](n). The primary structure of these materials represents the repetitive crystalline region of Bombyx mori silk fibroins incorporated with an elastic motif selected from animal elastin. The oligonucleotides were designed to encode the desired recombinant proteins and then expressed in the Escherichi coli system. The expression and purification conditions for the production of the recombinant proteins were optimized. (13)C CP/MAS NMR was used for structural characterization in the solid state, where the isotope labeling was performed using a modified M9 medium. The secondary structures of these materials are primarily governed by the designated amino acid sequence, where the B. mori silk fibroin block, (GAGAGS)(3), tends to form the crystalline region, which is interrupted by the flexible (GVPGV)(2) block. The CD data suggested that the structure of these materials was length-dependent in the solution state, i.e., a higher molecule weight leads to a higher ordered structure.

Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Publishing Authors By Initials

J Yao,T Asakura,

For similar macromolecular substances: polymers: biopolymers: silk research abstracts see: macromolecular substances: polymers: biopolymers: silk research

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Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 133

Page Numbers: 147-54

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2003

Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Keywords Mesh Terms:

KEYWORDS: Silk

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Synthesis and structural characterization of silk-like materials incorporated with an elastic motif. Information

Substance Name: Fibroins

Registry Number: 9007-76-5

Grant and Affiliation Information for Synthesis and structural characterization of silk-like materials incorporated with an elastic motif.

AFFILIATION: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588.

Country: Japan

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MEDLINETA: J Biochem

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