Synthesis and application of 2'-fluoro-substituted cap analogs.

Synthesis and application of 2'-fluoro-substituted cap analogs. Research Abstract Details 

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Synthesis and application of 2'-fluoro-substituted cap analogs. Abstract Text:

Anilkumar R Kore,Muthian Shanmugasundaram,Irudaya Charles,Angie M Cheng,Timothy J Barta,Anilkumar R Kore,Muthian Shanmugasundaram,Irudaya Charles,Angie M Cheng,Timothy J Barta,

Design, synthesis, and biological evaluation of 2'-fluoro-substituted cap analogs, i.e., m(7,2'F)G[5']ppp[5']G and m(7,2'F)G[5']ppp[5']m(7)G are described. Structures were confirmed by (1)H, (31)P, (19)F NMR and MS data. The effects of the 2'-fluoro-substituted moiety from the normal and N(7) double methylated mCAP were evaluated with respect to their capping efficiency, in vitro T7 RNA polymerase transcription efficiency, and translation activity using cultured HeLa cells. Luciferase fusion protein production was monitored by measuring the luciferase activity. mRNA poly(A) capped with 2'-fluoro-substituted cap analogs, (m(7,2'F)G[5']ppp[5']G) and (m(7,2'F)G[5']ppp[5']m(7)G), were translated approximately 2.4- and 2.5-fold more efficiently, respectively, than mRNA capped with conventional m(7)G[5']ppp[5']G.

Synthesis and application of 2'-fluoro-substituted cap analogs. Publishing Authors By Initials

AR Kore,M Shanmugasundaram,I Charles,AM Cheng,TJ Barta,AR Kore,M Shanmugasundaram,I Charles,AM Cheng,TJ Barta,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Synthesis and application of 2'-fluoro-substituted cap analogs. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioorganic & medicinal chemistry letters

VOLUME: 17

Page Numbers: 5295-9

Journal Abbreviation: Bioorg. Med. Chem. Lett.

ISSN: 0960-894X

DAY: 16

MONTH: 08

YEAR: 2007

Synthesis and application of 2'-fluoro-substituted cap analogs. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107377

Synthesis and application of 2'-fluoro-substituted cap analogs. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: Synthesis and application of 2'-fluoro-substituted cap analogs. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Synthesis and application of 2'-fluoro-substituted cap analogs.

AFFILIATION: Ambion Inc., An Applied Biosystems Business, Bioorganic Chemistry Division, 2130 Woodward Street, Austin, TX 78744-1832, USA. anil.kore@appliedbiosystems.com

Country: England

AGENCY: United States NIGMS

GRANT: R44GM070156-02

ACRONYM: GM

MEDLINETA: Bioorg Med Chem Lett

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