Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology.

Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Research Abstract Details 

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Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Abstract Text:

Guan-Sheng Jiao,Lars H Thoresen,Taeg Gyum Kim,Wade C Haaland,Feng Gao,Michael R Topp,Robin M Hochstrasser,Michael L Metzker,Kevin Burgess,

We have designed fluorescent "through-bond energy-transfer cassettes" that can harvest energy of a relatively short wavelength (e.g., 490 nm), and emit it at appreciably longer wavelengths without significant loss of intensity. Probes of this type could be particularly useful in biotechnology for multiplexing experiments in which several different outputs are to be observed from a single excitation source. Cassettes 1-4 were designed, prepared, and studied as model systems to achieve this end. They were synthesized through convergent routes that feature coupling of specially prepared fluorescein- and rhodamine-derived fragments. The four cassettes were shown to emit strongly, with highly efficient energy transfer. Their emission maxima cover a broad range of wavelengths (broader than the four dye cassettes currently used for most high-throughput DNA sequencing), and they exhibit faster energy-transfer rates than a similar through-space energy-transfer cassette. Specifically, energy-transfer rates in these cassettes is around 6-7 ps, in contrast to a similar through-space energy-transfer system shown to have a decay time of around 35 ps. Moreover, the cassettes are considerably more stable to photobleaching than fluorescein, even though they each contain fluorescein-derived donors. This was confirmed by bulk fluorescent measurements, and in single-molecule-detection studies. Modification of a commercial automated DNA-sequencing apparatus to detect the emissions of these four energy-transfer cassettes enabled single-color dye-primer sequencing.

Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Publishing Authors By Initials

GS Jiao,LH Thoresen,TG Kim,WC Haaland,F Gao,MR Topp,RM Hochstrasser,ML Metzker,K Burgess,

For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research

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Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chemistry (Weinheim an der Bergstrasse, Germany)

VOLUME: 12

Page Numbers: 7816-26

Journal Abbreviation:

ISSN: 0947-6539

DAY: 16

MONTH: Oct

YEAR: 2006

Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Information

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LANGUAGE: eng

NlmUniqueID: 9513783

Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Keywords Mesh Terms:

KEYWORDS: Spectrophotometry, Ultraviolet

MESH TERMS: methods

Chemical & Substance for Abstract: Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Information

Substance Name: Molecular Probes

Registry Number: 0

Grant and Affiliation Information for Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology.

AFFILIATION: Department of Chemistry, Texas A & M University P.O. Box 30012, College Station, Texas 77842, USA.

Country: Germany

AGENCY: United States NHGRI

GRANT: HG 01745

ACRONYM: HG

MEDLINETA: Chemistry

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