Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling.

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Abstract Text:

Eiki Koyama,Takanaga Ochiai,Ryan B Rountree,David M Kingsley,Motomi Enomoto-Iwamoto,Masahiro Iwamoto,Maurizio Pacifici,Eiki Koyama,Takanaga Ochiai,Ryan B Rountree,David M Kingsley,Motomi Enomoto-Iwamoto,Masahiro Iwamoto,Maurizio Pacifici,Eiki Koyama,Takanaga Ochiai,Ryan B Rountree,David M Kingsley,Motomi Enomoto-Iwamoto,Masahiro Iwamoto,Maurizio Pacifici,

Indian hedgehog (Ihh) has been previously found to regulate synovial joint formation. To analyze mechanisms, we carried out morphological, molecular, and cell fate map analyses of interzone and joint development in wild-type and Ihh(-/-) mouse embryo long bones. We found that Ihh(-/-) cartilaginous digit anlagen remained fused and lacked interzones or mature joints, whereas wrist skeletal elements were not fused but their joints were morphologically abnormal. E14.5 and E17.5 wild-type digit and ankle prospective joints expressed hedgehog target genes including Gli1 and Gli2 and interzone-associated genes including Gdf5, Erg, and tenascin-C, but expression of all these genes was barely detectable in mutant joints. For cell fate map analysis of joint progenitor cells, we mated Gdf5-Cre(+/-)/Rosa R26R(+/-) double transgenic mice with heterozygous Ihh(+/-) mice and monitored reporter beta-galactosidase activity and gene expression in triple-transgenic progeny. In control Gdf5-Cre(+/-)/R26R(+/-)/Ihh(+/-) limbs, reporter-positive cells were present in developing interzones, articulating layers, and synovial lining tissue and absent from underlying growth plates. In mutant Gdf5-Cre(+/-)/R26R(+/-)/Ihh(-/-) specimens, reporter-positive cells were present also. However, the cells were mostly located around the prospective and uninterrupted digit joint sites and, interestingly, still expressed Erg, tenascin-C, and Gdf5. Topographical analysis revealed that interzone and associated cells were not uniformly distributed, but were much more numerous ventrally. A similar topographical bias was seen for cavitation process and capsule primordia formation. In sum, Ihh is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal, but their patterning at prospective joint sites still occurs. The joint-forming functions of the cells appear to normally involve a previously unsuspected asymmetric distribution along the ventral-to-dorsal plane of the developing joint.

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Publishing Authors By Initials

E Koyama,T Ochiai,RB Rountree,DM Kingsley,M Enomoto-Iwamoto,M Iwamoto,M Pacifici,E Koyama,T Ochiai,RB Rountree,DM Kingsley,M Enomoto-Iwamoto,M Iwamoto,M Pacifici,E Koyama,T Ochiai,RB Rountree,DM Kingsley,M Enomoto-Iwamoto,M Iwamoto,M Pacifici,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Annals of the New York Academy of Sciences

VOLUME: 1116

Page Numbers: 100-12

Journal Abbreviation: Ann. N. Y. Acad. Sci.

ISSN: 0077-8923

DAY: 17

MONTH: Dec

YEAR: 2007

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7506858

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling.

AFFILIATION: Department of Orthopaedic Surgery, Thomas Jefferson University College of Medicine, 501 Curtis Bldg., 1015 Walnut Street, Philadelphia, PA 19107. maurizio.pacifici@jefferson.edu.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Ann N Y Acad Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling Related Publications

• A distinct cohort of progenitor cells participates in synovial joint and articular cartilage formation during mouse limb skeletogenesis.
• Conditional Kif3a ablation causes abnormal hedgehog signaling topography, growth plate dysfunction, and excessive bone and cartilage formation during mouse skeletogenesis.
• Estrogen deficiency, T cells and bone loss.
• Indian and sonic hedgehogs regulate synchondrosis growth plate and cranial base development and function.
• Oxidative stress causes bone loss in estrogen-deficient mice through enhanced bone marrow dendritic cell activation.
• Synovial Joint Formation during Mouse Limb Skeletogenesis: Roles of Indian Hedgehog Signaling.
• T cells potentiate PTH-induced cortical bone loss through CD40L signaling.
• T cells: unexpected players in the bone loss induced by estrogen deficiency and in Basal bone homeostasis.
• [Epidemiological surveillance for infectious diseases and humanitarian emergencies]
• [Infectious diseases and population assistance: general issues]
• [Laws relevant to international missions of health cooperation]
• [New global challenges: the role of international organizations]
• [Optimizing international humanitarian assistance through the definition of personal profiles: the role of graphology]
• [Probability of the functional vestibulo-oculo-cervico-spinal ways affecting the placement and maintenance of the body's center of gravity in the support plane in erect man. A statokinesimetric study]