Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells.

Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Research Abstract Details 

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Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Abstract Text:

Younghee Lee,Hal E Broxmeyer,Charlie Mantel,Hyung-Joo Kwon,Jae Wha Kim,Jin Sook Kim,Durhan Kwon,In Seong Choe,

Stem cell factor (SCF) is an early-acting cytokine inducing proliferative synergy with other cytokines in hematopoietic cells. We earlier showed that p21 was synergistically induced in SCF synergy and the p44/42 MAPK pathway was essential for the transcriptional control of p21. SCF synergy accompanies protein synthesis. p70S6K implicated in translational control in many other systems has not been shown in SCF synergy induced system. GM-CSF dependent human cell line MO7e was stimulated with GM-CSF with SCF, and investigated activation of p70S6K by using phospho-specific antibody. A possible contribution of p70S6K to SCF synergy was examined by measuring p21 induction as a model system. p70S6K was slightly activated by GM-CSF alone and markedly activated by SCF alone. Combined stimulation with these two cytokines synergistically activated p70S6K resulting in persistent activation. Addition of the pathway-specific inhibitors for P13K or FRAP/TOR, two up-stream pathways of p70S6K resulted in abolishment of p70S6K phosphorylation and also significant reduction of p21 protein level. These data suggest that synergistically activated p70S6K by GM-CSF plus SCF involves, at least in part, protein translational control including regulation of p21 protein.

Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Publishing Authors By Initials

Y Lee,HE Broxmeyer,C Mantel,HJ Kwon,JW Kim,JS Kim,D Kwon,IS Choe,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: p21-activated kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: p21-activated kinases research

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Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Experimental & molecular medicine

VOLUME: 35

Page Numbers: 222-6

Journal Abbreviation: Exp. Mol. Med.

ISSN: 1226-3613

DAY: 30

MONTH: Jun

YEAR: 2003

Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9607880

Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Keywords Mesh Terms:

KEYWORDS: p21-Activated Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells. Information

Substance Name: Tacrolimus Binding Protein 1A

Registry Number: EC 5.2.1.-

Grant and Affiliation Information for Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells.

AFFILIATION: Cell Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Daejon 305-600, Korea.

Country: Korea (South)

AGENCY: United States NHLBI

GRANT: R01 HL 67384

ACRONYM: HL

MEDLINETA: Exp Mol Med

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