Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation.

Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Research Abstract Details 

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Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Abstract Text:

Quan-Jiang Zhang,Qiu-Xia Li,Hai-Feng Zhang,Kun-Ru Zhang,Wen-Yi Guo,Hai-Chang Wang,Zhuan Zhou,He-Ping Cheng,Jun Ren,Feng Gao,

OBJECTIVES: Physical activity has been well known to benefit heart function. The improved autonomic nervous activity is considered to be mainly responsible for this beneficial effect. However, the precise mechanism behind the intrinsic myocardial responsiveness to exercise is still unclear. This study was designed to examine the effect of swim training on myocardial response to insulin with a special focus on the endogenous endothelial nitric oxide synthase (eNOS)-nitric oxide (NO) cascade. METHODS: Adult male Sprague-Dawley (SD) rats were subjected to a 10-week free-loading swim training (3 h/day, 5 days/week). Contractile response to insulin at the levels of cardiomyocytes and isolated perfused heart, myocardial glucose uptake and post-insulin receptor signaling cascades were evaluated. RESULTS: Swim training enhanced cardiac contractile response to insulin in cardiomyocytes and isolated perfused heart, respectively. The improved cardiac response was accompanied by facilitated insulin-stimulated glucose uptake, GLUT4 translocation and upregulation of Akt and eNOS expression (p<0.01). Treatment with insulin resulted in a 3.6- and 2.2-fold increase of eNOS phosphorylation (p<0.01), as well as a 3.0- and 1.9-fold increase of Akt phosphorylation in exercise and sedentary groups, respectively (p<0.01). In addition, exercise significantly facilitated insulin-induced myocardial NO production (p<0.01 vs. sedentary). Moreover, pretreatment with either LY294002, a phosphatidylinositol-3 kinase (PI-3K) inhibitor or L-NAME, a NOS inhibitor, abolished the exercise-induced sensitization of myocardial contractile response to insulin, insulin-induced NO production and phosphorylation of Akt and eNOS. CONCLUSION: These results demonstrate that swim training is capable of sensitizing myocardial contractile response to insulin via upregulation of Akt- and eNOS signaling cascades.

Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Publishing Authors By Initials

QJ Zhang,QX Li,HF Zhang,KR Zhang,WY Guo,HC Wang,Z Zhou,HP Cheng,J Ren,F Gao,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

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Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cardiovascular research

VOLUME: 75

Page Numbers: 369-80

Journal Abbreviation: Cardiovasc. Res.

ISSN: 0008-6363

DAY: 4

MONTH: 05

YEAR: 2007

Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Information

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LANGUAGE: eng

NlmUniqueID: 77427

Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: metabolism

Chemical & Substance for Abstract: Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation. Information

Substance Name: Proto-Oncogene Proteins c-akt

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Swim training sensitizes myocardial response to insulin: role of Akt-dependent eNOS activation.

AFFILIATION: Department of Physiology, Xijing Hospital, Fourth Military Medical University, Xi' an 710032, China.

Country: Netherlands

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MEDLINETA: Cardiovasc Res

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