Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Abstract Text:

Paul R Avery,Tracy L Lehman,Edward A Hoover,Steven W Dow,

Currently most dendritic cells (DC) for in vitro study are generated from bone marrow or peripheral blood by culture in high concentrations of GM-CSF and other cytokines. However, in mice it is also possible to generate DC from spleen cells using long-term stromal cell cultures. To determine whether tissue DC could be also be generated from cats, we established stromal cell cultures from a number of different tissues of newborn cats. We found that stromal cell cultures from spleen, lung, liver, kidney, brain, and lymph node tissues were all capable of spontaneously generating DC over long periods of time (months), without requiring the addition of exogenous cytokines. The tissue DC generated from these stromal cell cultures could be readily isolated at high purity by simple mechanical detachment. The feline tissue DC expressed high levels of CD11c, CD11b, and MHC Class II and variable levels of CD80 and CD14 and exhibited high levels of spontaneous macropinocytosis. Moreover, DC from spleen stromal cell cultures, but not DC from lung or liver stromal cell cultures, stimulated mixed-lymphocyte reactions. The DC generated from the stromal cell cultures were relatively independent of GM-CSF for survival and proliferation, indicative of a dependence on other growth factors produced by the stromal cells. These results suggest that tissues of young cats contain a population of resident DC progenitor cells that under appropriate conditions are capable of spontaneous proliferation and generation of immature DC.

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Publishing Authors By Initials

PR Avery,TL Lehman,EA Hoover,SW Dow,

For similar cells: connective tissue cells: stromal cells research abstracts see: cells: connective tissue cells: stromal cells research

PUBMED ID PMID:

MEDLINE DATE:

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Veterinary immunology and immunopathology

VOLUME: 117

Page Numbers: 222-35

Journal Abbreviation: Vet. Immunol. Immunopathol.

ISSN: 0165-2427

DAY: 16

MONTH: 02

YEAR: 2007

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8002006

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Keywords Mesh Terms:

KEYWORDS: Stromal Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures. Information

Substance Name: Granulocyte-Macrophage Colony-Stimulatin

Registry Number: 83869-56-1

Grant and Affiliation Information for Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.

AFFILIATION: Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft. Collins, CO 80523, United States.

Country: Netherlands

AGENCY: United States NCRR

GRANT: T32RR00707

ACRONYM: RR

MEDLINETA: Vet Immunol Immunopathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures Related Publications

• Action potentials of cerebellar cortex in response to local electrical stimulation.
• Carbon monoxide exposure on denali: comparing the 2004 and 2005 climbing seasons.
• Complementary global maps for orientation coding in upper and lower layers of the monkey's foveal striate cortex.
• Continuous low-dose oral chemotherapy for adjuvant therapy of splenic hemangiosarcoma in dogs.
• Corticotropin releasing hormone modulates endotoxin-induced inflammatory cytokine expression in human trophoblast cells.
• Diarrheal disease control studies; conical net for collecting flies.
• Diversification of the Function of Cell-to-Cell Signaling in Regulation of Virulence Within Plant Pathogenic Xanthomonads.
• EXTRINSIC REGULATORY MECHANISMS OF SEIZURE ACTIVITY.
• Evaluation of a novel tumor vaccine in dogs with hemangiosarcoma.
• Evaluation of a xenogeneic VEGF vaccine in dogs with soft tissue sarcoma.
• Excitability of skeletal muscle during development, denervation, and tissue culture.
• Foveal tracking cells in the superior temporal sulcus of the macaque monkey.
• Indications for steroid anaesthesia.
• Infants' early ability to segment the conversational speech signal predicts later language development: a retrospective analysis.
• Integrative physiology, functional genomics and the phenotype gap: a guide for comparative physiologists.
• Interspecies signalling via the Stenotrophomonas maltophilia diffusible signal factor influences biofilm formation and polymyxin tolerance in Pseudomonas aeruginosa.
• Intradural chordoma.
• Liposome-nucleic acid immunotherapeutics.
• Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin.
• Neutrophil function in septic dogs.
• Oxidative stress during acute FIV infection in cats.
• Permanent transvenous dual-chamber pacing using the coronary sinus in a patient with a mechanical prosthetic tricuspid valve.
• Stance dependence of automatic postural adjustments in humans.
• Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
• The effect of prolonged veno-arterial pumping on the circulation of the dog.
• The establishment clause argument for choice.
• Treatment of cerebral palsy; clinical study in use of d-tubocurarine in peanut oil and myricin.
• Use of FoxP3 expression to identify regulatory T cells in healthy dogs and dogs with cancer.
• Use of radium in treatment of hypertrophic lymphoid tissue in the nasopharynx.
• Vaccination with liposome--DNA complexes elicits enhanced antitumor immunity.