Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma.

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Abstract Text:

Zeng Bian Zhu,Yabing Chen,Sharmila K Makhija,Baogen Lu,Minghui Wang,Angel A Rivera,Masato Yamamoto,Shuyi Wang,Gene P Siegal,David T Curiel,Jay M McDonald,

Cholangiocarcinoma is a highly malignant neoplasm with no effective treatment. Conditionally replicative adenoviruses (CRAds) represent a promising new modality for the treatment of cancer in general. A key contribution in this regard was the introduction of tumor-selective viral replication for amplification of the initial inoculum in the neoplastic cell population. Under ideal conditions following cellular infection, the viruses replicate selectively in the infected tumor cells and kill the cells by cytolysis, leaving normal cells unaffected. However, to date there have been two limitations to the clinical application of these CRAd agents, i.e. poor viral infectivity and tumor specificity. Here we report the construction of three new CRAd agents, CRAd-S.RGD, CRAd-S.F5/3 and CRAd-S.pk7, in which the tumor specificity is regulated by a tumor-specific promoter, the survivin promoter, and the viral infectivity is enhanced by incorporating a capsid modification (RGD, F5/3 or pk7) in the adenovirus fiber region. These CRAd agents effectively target cholangiocarcinoma cells, induce strong cytoxicity in these cells in vitro and inhibit tumor growth in a murine xenograft model in vivo. In addition, the survivin promoter has extremely low activity both in the non-transformed cell line, HMEC, and in human liver tissue. Our results suggest that the survivin-based CRAds are promising agents for targeting cholangiocarcinoma with low host toxicity. Such results should provide important insights into the identification of novel therapeutic strategies for cholangiocarcinoma.

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Publishing Authors By Initials

ZB Zhu,Y Chen,SK Makhija,B Lu,M Wang,AA Rivera,M Yamamoto,S Wang,GP Siegal,DT Curiel,JM McDonald,

For similar xenograft model antitumor assays research abstracts see: xenograft model antitumor assays research

PUBMED ID PMID:

MEDLINE DATE:

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International journal of oncology

VOLUME: 29

Page Numbers: 1319-29

Journal Abbreviation: Int. J. Oncol.

ISSN: 1019-6439

DAY: 3

MONTH: Nov

YEAR: 2006

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9306042

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Keywords Mesh Terms:

KEYWORDS: Xenograft Model Antitumor Assays

MESH TERMS: genetics

Chemical & Substance for Abstract: Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma. Information

Substance Name: Neoplasm Proteins

Registry Number: 0

Grant and Affiliation Information for Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma.

AFFILIATION: Division of Human Gene Therapy, Department of Medicine, Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35291, USA.

Country: Greece

AGENCY: United States NIDDK

GRANT: R01 DK063615-01

ACRONYM: DK

MEDLINETA: Int J Oncol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma Related Publications

• A computerized decision support system improves the accuracy of temperature capture from nursing personnel at the bedside.
• A novel subpopulation of 5-HT type 3A receptor subunit immunoreactive interneurons in the rat basolateral amygdala.
• A randomized, placebo-controlled trial of oral beclomethasone dipropionate as a prednisone-sparing therapy for gastrointestinal graft-versus-host disease.
• Acute interhemispheric subdural haematoma.
• Atlanto-occipital and atlanto-axial instability in children with Down syndrome.
• Automated mapping of local radiology terms to LOINC.
• Ayurveda and acupuncture in heroin detoxification in Sri Lanka.
• Chronic kidney disease in long-term survivors of hematopoietic cell transplant.
• Computerized reminders to improve isolation rates of patients with drug-resistant infections: design and preliminary results.
• Conducting Translation Research in the Home Care Setting: Lessons from a Just-in-Time Reminder Study.
• Efficiency, comprehensiveness and cost-effectiveness when comparing dictation and electronic templates for operative reports.
• Es07 microarray gene expression analysis of human adrenocortical tumours.
• Examination of the mouth.
• Extensible Stylesheet Language Formatting Objects (XSL-FO): a tool to transform patient data into attractive clinical reports.
• Fischer carbene catalysis of alkynol cycloisomerization: application to the synthesis of the altromycin B disaccharide.
• Functional significance of Asn-linked glycosylation of proteinase 3 for enzymatic activity, processing, targeting, and recognition by anti-neutrophil cytoplasmic antibodies.
• Group a streptococcus osteomyelitis and septic arthritis following varicella: Case report and review of the literature.
• Human serum amyloid A3 peptide enhances intestinal MUC3 expression and inhibits EPEC adherence.
• Impaired spatial learning in the APPSwe + PSEN1DeltaE9 bigenic mouse model of Alzheimer's disease.
• Impaired spatial memory in APP-overexpressing mice on a homocysteinemia-inducing diet.
• In support of emergency department health information technology.
• Physiochemical properties of oviductal fluid from rabbits exposed to heat stress.
• Rapid vascular regrowth in tumors after reversal of VEGF inhibition.
• S641 contributes HERG k+ channel inactivation.
• Sarcomere length dependence of rat skinned cardiac myocyte mechanical properties: dependence on myosin heavy chain.
• Sexual behavior of farm animals.
• Spatial and nonspatial escape strategies in the Barnes maze.
• Survivin promoter-based conditionally replicative adenoviruses target cholangiocarcinoma.
• Targeted adenoviral gene transfer to the kidney.
• Tissue-sparing effect of x-ray microplanar beams particularly in the CNS: is a bystander effect involved?