Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus.

Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus. Research Abstract Details 

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Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus. Abstract Text:

D I Jin,R M Petters,B H Johnson,R M Shuman,

To determine the relative survival of porcine embryos after co-culture with cells producing an avian retrovirus, four-cell stage embryos were obtained from sows following synchronization with altrenogest and superovulation with gonadotropins. These embryos were randomly assigned to the following treatments: no manipulation (zona-intact); zona removed with acidified Tyrode's solution (zona-free); and zona removed followed by co-culture with D-17 canine cells producing an avian retrovirus vector derived from spleen necrosis virus (zona-free + co-culture). The survival rates of four-cell stage embryos to morulae or early blastocysts during a 48-h culture period were 93.3, 80.0 and 57.7% in zona-intact, zona-free and zona-free + co-culture groups, respectively. Following embryo transfer, the development of embryos to fetuses at six weeks of gestation was 37.5, 30.0 and 11.7% in zona-intact, zona-free and zona-free + co-culture groups. These results indicate that early preimplantation porcine embryos can develop to apparently normal fetuses following co-culture with cells producing a retrovirus, and the feasibility of this method for retrovirus-mediated gene transfer in pigs was demonstrated.

Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus. Publishing Authors By Initials

DI Jin,RM Petters,BH Johnson,RM Shuman,

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Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 35

Page Numbers: 521-6

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Mar

YEAR: 1991

Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Survival of early preimplantation porcine embryos after co-culture with cells producing an avian retrovirus.

AFFILIATION: Reproductive Physiology Research Laboratory Department of Animal Science North Carolina State University Raleigh, NC 27695-7621 USA.

Country: United States

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MEDLINETA: Theriogenology

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