Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype.

Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype. Research Abstract Details 

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Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype. Abstract Text:

Yukiko Hara-Kudo,Jun Niizuma,Ikuo Goto,Shinji Iizuka,Yoshifumi Kaji,Kazumasa Kamakura,Sosuke Suzuki,Kosuke Takatori,

To detect various serotypes of Shiga toxin-producing Escherichia coli (STEC) in food, methods independent of serotyping are needed. We established procedures to isolate STEC using a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay targeting the Shiga toxin (ST) gene and a method of plating LAMP assay positive dilutions onto media for the selection of E. coli. After incubation, suspensions of a colony or some colonies were tested in the LAMP assay. Positive suspensions were diluted and plated onto selective media. The procedure was repeated. Finally, LAMP positive colonies were confirmed as STEC and serotype. As a result of surveillance in beef in 2005-2007, 11 of 720 samples (1.5%) tested positive for the ST gene by LAMP assay. Serotype O8, O128, and O-untypeable STEC were isolated from the samples by the newly established procedure. It was demonstrated that the procedure was effective for detecting STEC independent of serotype.

Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype. Publishing Authors By Initials

Y Hara-Kudo,J Niizuma,I Goto,S Iizuka,Y Kaji,K Kamakura,S Suzuki,K Takatori,

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Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Foodborne pathogens and disease

VOLUME: 5

Page Numbers: 97-103

Journal Abbreviation: Foodborne Pathog. Dis.

ISSN: 1556-7125

DAY: 11

MONTH: 02

YEAR: 2008

Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype. Information

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LANGUAGE: eng

NlmUniqueID: 101120121

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AFFILIATION: Division of Microbiology, National Institute of Health Sciences, Tokyo, Japan. ykudo@nihs.go.jp

Country: United States

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MEDLINETA: Foodborne Pathog Dis

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