Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection.

Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Research Abstract Details 

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Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Abstract Text:

Lan Sun,Chenxu Yu,Joseph Irudayaraj,

To provide rapid and accurate detection of DNA markers in a straightforward, inexpensive, and multiplex format, an alternative surface-enhanced Raman scattering based probe was designed and fabricated to covalently attach both DNA probing sequence and nonfluorescent Raman tags to the surface of gold nanoparticles (DNA-AuP-RTag). The intensity of Raman signal of the probes could be controlled through the surface coverage of the nonfluorescent Raman tags (RTags). Detection sensitivity of these probes could be optimized by fine-tuning the amount of DNA molecules and RTags on the probes. Long-term stability of the DNA-AuP-RTag probes was found to be good (over 3 months). Excellent multiplexing capability of the DNA-AuP-RTag scheme was demonstrated by simultaneous identification of up to eight probes in a mixture. Detection of hybridization of single-stranded DNA to its complementary targets was successfully accomplished with a long-term goal to use nonfluorescent RTags in a Raman-based DNA microarray platform.

Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Publishing Authors By Initials

L Sun,C Yu,J Irudayaraj,

For similar transition temperature research abstracts see: transition temperature research

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Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 3981-8

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 28

MONTH: 04

YEAR: 2007

Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Keywords Mesh Terms:

KEYWORDS: Transition Temperature

MESH TERMS: methods

Chemical & Substance for Abstract: Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection.

AFFILIATION: Department of Agricultural and Biological Engineering and Bindley Bioscience Center (Physiological Sensing Facility), Purdue University, 225 South University Street, West Lafayette, Indiana 47907, USA.

Country: United States

AGENCY: United States NCI

GRANT: 1 R03 CA121347-01

ACRONYM: CA

MEDLINETA: Anal Chem

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