Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47.

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Abstract Text:

Zhiyin Xia,Katsushige Abe,Akira Furusu,Masanobu Miyazaki,Yoko Obata,Yasuhiko Tabata,Takehiko Koji,Shigeru Kohno,Zhiyin Xia,Katsushige Abe,Akira Furusu,Masanobu Miyazaki,Yoko Obata,Yasuhiko Tabata,Takehiko Koji,Shigeru Kohno,

BACKGROUND/AIM: Unilateral ureteral obstruction (UUO) is a well-established model for tubulointerstitial fibrosis. During the progression of tubulointerstitial fibrosis, upregulation of collagen synthesis and subsequent accumulation of collagen were observed in the tubulointerstitial area. Heat shock protein 47 (HSP47) is a collagen-specific molecular chaperone and plays an essential role in regulating collagen synthesis. We designed small interfering RNA (siRNA) sequences for HSP47 mRNA to examine whether HSP47 is involved in the progression of renal tubulointerstitial fibrosis in a mouse UUO model. METHODS: The HSP47 siRNA was injected once via the ureter at the time of UUO preparation. We also applied a new gene delivery system for siRNA using cationized gelatin microspheres. The kidneys were harvested 7 and 14 days after UUO. The HSP47 and type I, III, and IV collagen expression levels were analyzed by immunohistochemistry and Western blotting. RESULTS: Seven days after UUO, the expression levels of HSP47 and type I, III, and IV collagens were markedly upregulated in obstructed kidneys or green fluorescent protein siRNA treated obstructed kidneys. HSP47 siRNA injection significantly reduced the protein expression levels and significantly diminished the accompanying interstitial fibrosis. Moreover, cationized gelatin microspheres as a delivery system enhanced and lengthened the antifibrotic effect of HSP47 siRNA. CONCLUSIONS: Our results indicate that HSP47 is a candidate target for the prevention of tubulointerstitial fibrosis and that selective blockade of the HSP47 expression by using siRNA could be a potentially useful therapeutic approach for patients with renal disease.

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Publishing Authors By Initials

Z Xia,K Abe,A Furusu,M Miyazaki,Y Obata,Y Tabata,T Koji,S Kohno,Z Xia,K Abe,A Furusu,M Miyazaki,Y Obata,Y Tabata,T Koji,S Kohno,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of nephrology

VOLUME: 28

Page Numbers: 34-46

Journal Abbreviation: Am. J. Nephrol.

ISSN: 1421-9670

DAY: 21

MONTH: 09

YEAR: 2007

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109361

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47.

AFFILIATION: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Country: Switzerland

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Nephrol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47 Related Publications

• Acute hepatitis E of a man who consumed wild boar meat prior to the onset of illness in Nagasaki, Japan.
• Association of uncoupling protein-2 expression with increased reactive oxygen species in residual myocardium of the enlarged left ventricle after myocardial infarction.
• Bone morphogenetic protein-2 promotes survival and differentiation of striatal GABAergic neurons in the absence of glial cell proliferation.
• Cardiac function as assessed by echocardiography in the oldest old >/= 90 years of age.
• Circulating visfatin level is correlated with inflammation, but not with insulin resistance.
• Comparison of the grade evaluated by "Liver damage" of Liver Cancer Study Group of Japan and Child-Pugh classification in patients with hepatocellular carcinoma.
• Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase.
• Detection of new antigenic proteins in Japanese cedar pollen.
• Differential expression of the glycosylated forms of MUC1 during lung development.
• Effects of acetaldehyde on human airway constriction and inflammation.
• Expression of aldosterone synthase gene in IgA nephropathy (IgAN).
• Granulocyte colony-stimulating factor facilitates the angiogenesis induced by ultrasonic microbubble destruction.
• How transmembrane proteins sense endoplasmic reticulum stress.
• In vitro and in vivo activities of novel fluoroquinolones alone and in combination with clarithromycin against clinically isolated Mycobacterium avium complex strains in Japan.
• Intracellular balance of oxidative stress and cytoprotective molecules in damaged interlobular bile ducts in autoimmune hepatitis and primary biliary cirrhosis: In situ detection of 8-hydroxydeoxyguanosine and glutathione-S-transferase-pi.
• Latent cardiac dysfunction as assessed by echocardiography in bed-bound patients following cerebrovascular accidents.
• Low-dose oral methotrexate for the management of childhood Cogan's syndrome: a case report.
• Phase I study of irinotecan and gemcitabine in previously untreated patients with advanced non-small cell lung cancer.
• Recent advances in understanding the unfolded protein response.
• Serum marker KL-6/MUC1 for the diagnosis and management of interstitial pneumonitis.
• Signal transducer and activator of transcription 3 involvement in the development of renal interstitial fibrosis after unilateral ureteral obstruction.
• Specific activation of the p38 mitogen-activated protein kinase signaling pathway and induction of neurite outgrowth in PC12 cells by bone morphogenetic protein-2.
• Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens.
• Suppression of renal tubulointerstitial fibrosis by small interfering RNA targeting heat shock protein 47.
• Telephone counseling of athletes abusing anabolic-androgenic steroids.
• Transgenic mice expressing a fully nontoxic diphtheria toxin mutant, not CRM197 mutant, acquire immune tolerance against diphtheria toxin.
• Wireless Vital Sign Monitoring using Ultra Wideband-Based Personal Area Networks.
• [A case of idiopathic chylothorax successfully treated with surgical ligation of thoracic ducts]
• [A case of mycoplasmal pneumonia with bronchiolitis treated with steroids]
• [Nontuberculous mycobacterial infections involving solitary pulmonary nodules]