Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides.

Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides. Research Abstract Details 

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Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides. Abstract Text:

Eui-Jin Kim,Ju-Sim Kim,Il-Han Lee,Hae J Rhee,Jeong K Lee,

Chlorophyllide a reductase of Rhodobacter sphaeroides, which were reconstituted with the purified subunits of BchX, BchY, and BchZ, reduced ring B of chlorophyllide a using NADH under anaerobic conditions. Interestingly, suppressor mutations rescuing the inability of R. sphaeroides Fe-SOD mutant to grow in succinate-based minimal medium were predominantly mapped to BchZ subunit of chlorophyllide a reductase. The enzyme is labile in the presence of O(2). However, it generates superoxide at low O(2). The enzymes reconstituted with BchX, BchY, and the mutein subunit of BchZ from suppressor mutants showed less activity not only for chlorophyllide a reduction but also for superoxide generation compared with the enzyme reconstituted with the wild-type subunits. BchX, which contains FMN, and BchY are iron-sulfur proteins, whereas BchZ is a hemoprotein containing b-type heme. Neither chlorophyllide a reduction nor superoxide generation was observed with the enzyme reconstituted with the wild-type subunits of BchX and BchY, and the apo-subunit of BchZ that had been refolded without heme, in which FMN of BchX was fully reduced. Thus, superoxide is generated not from FMN of BchX but from heme of BchZ. Consistently, the heme of BchZ muteins was half-reduced in its redox state compared with that of wild-type BchZ.

Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides. Publishing Authors By Initials

EJ Kim,JS Kim,IH Lee,HJ Rhee,JK Lee,

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Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 3718-30

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 12

MONTH: 12

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea.

Country: United States

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MEDLINETA: J Biol Chem

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