Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis.

Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Research Abstract Details 

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Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Abstract Text:

B J Van Klinken,J W Van der Wal,A W Einerhand,H A ,J Dekker,

BACKGROUND: Decreased synthesis of the predominant secretory human colonic mucin (MUC2) occurs during active ulcerative colitis. AIMS: To study possible alterations in mucin sulphation and mucin secretion, which could be the cause of decreased mucosal protection in ulcerative colitis. METHODS: Colonic biopsy specimens from patients with active ulcerative colitis, ulcerative colitis in remission, and controls were metabolically labelled with [35S]-amino acids or [35S]-sulphate, chase incubated and analysed by SDS-PAGE, followed by quantitation of mature [35S]-labelled MUC2. For quantitation of total MUC2, which includes non-radiolabelled and radiolabelled MUC2, dot blotting was performed, using a MUC2 monoclonal antibody. RESULTS: Between patient groups, no significant differences were found in [35S]-sulphate content of secreted MUC2 or in the secreted percentage of either [35S]-amino acid labelled MUC2 or total MUC2. During active ulcerative colitis, secretion of [35S]-sulphate labelled MUC2 was significantly increased twofold, whereas [35S]-sulphate incorporation into MUC2 was significantly reduced to half. CONCLUSIONS: During active ulcerative colitis, less MUC2 is secreted, because MUC2 synthesis is decreased while the secreted percentage of MUC2 is unaltered. Furthermore, sulphate content of secreted MUC2 is unaltered by a specific compensatory mechanism, because sulphated MUC2 is preferentially secreted while sulphate incorporation into MUC2 is reduced.

Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Publishing Authors By Initials

BJ Van Klinken,JW Van der Wal,AW Einerhand,HA ,J Dekker,

For similar inorganic chemicals: isotopes: radioisotopes: sulfur radioisotopes research abstracts see: inorganic chemicals: isotopes: radioisotopes: sulfur radioisotopes research

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Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Gut

VOLUME: 44

Page Numbers: 387-93

Journal Abbreviation: Gut

ISSN: 0017-5749

DAY: 30

MONTH: Mar

YEAR: 1999

Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Information

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LANGUAGE: eng

NlmUniqueID: 2985108

Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Keywords Mesh Terms:

KEYWORDS: Sulfur Radioisotopes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. Information

Substance Name: Sulfur Radioisotopes

Registry Number: 0

Grant and Affiliation Information for Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis.

AFFILIATION: Pediatric Gastroenterology and Nutrition, Department of Pediatrics, University of Amsterdam, Academic Medical Centre, Amsterdam, The Netherlands.

Country: ENGLAND

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MEDLINETA: Gut

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