Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects.

Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Research Abstract Details 

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Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Abstract Text:

Melinda C Myzak,Philip Tong,Wan-Mohaiza Dashwood,Roderick H Dashwood,Emily Ho,

Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables such as broccoli. This anticarcinogen was first identified as a potent inducer of Phase 2 enzymes, but evidence is mounting that SFN acts through other cancer chemopreventive mechanisms. We recently reported on a novel mechanism of chemoprotection by SFN in human colon cancer cells and prostate epithelial cells, namely the inhibition of histone deacetylase (HDAC). In the present investigation, we sought to test whether SFN also might inhibit HDAC activity in vivo. When consumed in the diet at an average daily dose of 7.5 mumol per animal for 21 days, SFN suppressed the growth of human PC-3 prostate cancer cells by 40% in male nude mice. There was a significant decrease in HDAC activity in the xenografts, as well as in the prostates and mononuclear blood cells (MBC), of mice treated with SFN, compared to controls. There also was a trend towards increased global histone acetylation in the xenografts, prostates, and MBC. In human subjects, a single dose of 68 g BroccoSprouts inhibited HDAC activity significantly in peripheral blood mononuclear cells (PBMC) 3 and 6 hrs following consumption. These findings provide evidence that one mechanism through which SFN acts as a cancer chemopreventive agent in vivo is through the inhibition of HDAC activity. Moreover, the data suggest that HDAC activity in PBMC may be used as a biomarker for assessing exposure to novel dietary HDAC inhibitors in human subjects.

Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Publishing Authors By Initials

MC Myzak,P Tong,WM Dashwood,RH Dashwood,E Ho,

For similar surgical procedures, operative: transplantation: transplantation, heterologous research abstracts see: surgical procedures, operative: transplantation: transplantation, heterologous research

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Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental biology and medicine (Maywood, N.J.)

VOLUME: 232

Page Numbers: 227-34

Journal Abbreviation: Exp. Biol. Med. (Maywood)

ISSN: 1535-3702

DAY: 3

MONTH: Feb

YEAR: 2007

Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Information

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LANGUAGE: eng

NlmUniqueID: 100973463

Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Keywords Mesh Terms:

KEYWORDS: Transplantation, Heterologous

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects. Information

Substance Name: Histone Deacetylases

Registry Number: EC 3.5.1.-

Grant and Affiliation Information for Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects.

AFFILIATION: Linus Pauling Institute, Molecular and Cellular Biology Program, Oregon State University, Corvallis, Oregon 97331, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: P30 ES00210

ACRONYM: ES

MEDLINETA: Exp Biol Med (Maywood)

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