Subunit Vaccine against the Seven Serotypes of Botulism.

Subunit Vaccine against the Seven Serotypes of Botulism. Research Abstract Details 

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Subunit Vaccine against the Seven Serotypes of Botulism. Abstract Text:

Michael R Baldwin,William H Tepp,Amanda Przedpelski,Christina L Pier,Marite Bradshaw,Eric A Johnson,Joseph T Barbieri,

Botulinum neurotoxins (BoNTs) are the most toxic proteins for humans and are classified as category A toxins. There are seven serotypes of BoNTs defined by the lack of cross-serotype toxin neutralization. Thus, an effective vaccine must neutralize each BoNT serotype. BoNTs are organized as dichain A-B toxins, where the N-terminal domain (light chain) is a zinc metalloprotease targeting soluble NSF attachment receptor proteins that is linked to the C-terminal domain (heavy chain [HC]) by a disulfide bond. The HC comprises a translocation domain and a C-terminal receptor binding domain (HCR). HCRs of the seven serotypes of BoNTs (hepta-HCR) were engineered for expression in Escherichia coli, and each HCR was purified from E. coli lysates. Immunization of mice with the E. coli-derived hepta-serotype HCR vaccine elicited an antibody response to each of the seven BoNT HCRs and neutralized challenge by 10,000 50% lethal doses of each of the seven BoNT serotypes. A solid-phase assay showed that the anti-hepta-serotype HCR sera inhibited the binding of HCR serotypes A and B to the ganglioside GT1b, the first step in BoNT intoxication of neurons. This is the first E. coli-derived vaccine that effectively neutralizes each of the seven BoNT serotypes.

Subunit Vaccine against the Seven Serotypes of Botulism. Publishing Authors By Initials

MR Baldwin,WH Tepp,A Przedpelski,CL Pier,M Bradshaw,EA Johnson,JT Barbieri,

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Subunit Vaccine against the Seven Serotypes of Botulism. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Infection and immunity

VOLUME: 76

Page Numbers: 1314-8

Journal Abbreviation: Infect. Immun.

ISSN: 1098-5522

DAY: 10

MONTH: 12

YEAR: 2007

Subunit Vaccine against the Seven Serotypes of Botulism. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Subunit Vaccine against the Seven Serotypes of Botulism.

AFFILIATION: Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226. jtb01@mcw.edu.

Country: United States

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MEDLINETA: Infect Immun

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