Substrate specificity of nuclease TT1 from Thermus thermophilus HB8.

Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Research Abstract Details 

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Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Abstract Text:

M Takahashi,

The substrate and the action mechanism of a nuclease named nuclease TT1, from the culture broth of an extreme thermophile, Thermus thermophilus HB8, were investigated. The enzyme is nonspecific for the sugar moiety and cleaves both single- and double-stranded DNAs, rRNA, tRNA and oligonucleotides irrespective of chain length to produce 5'-mononucleotides exonucleolitically. The action mechanism is processive and the enzyme shows no porality of degradation. The minimal unit as a substrate is a 5'-dinucleotide. The rate of hydrolysis is independent of a terminal phosphate group. The substrate lacking a 5'-phosphoryl group is degraded to leave the 5'-terminus and the penultimate nucleotide (NpN) as a core. The substrate possessing a 3'-phosphoryl group is degraded to leave the mononucleoside 5',3'-diphosphates (pNp). However, NpN and pNp are gradually degraded by a large dose of the enzyme to produce a 5'-mononucleotide. The enzyme is free from nonspecific phosphatase and phosphodiesterase activities. Application of this enzyme to determine the sequence of oligonucleotides is shown.

Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Publishing Authors By Initials

M Takahashi,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus research

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Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 88

Page Numbers: 59-68

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1980

Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Keywords Mesh Terms:

KEYWORDS: Thermus

MESH TERMS: enzymology

Chemical & Substance for Abstract: Substrate specificity of nuclease TT1 from Thermus thermophilus HB8. Information

Substance Name: nuclease TT1

Registry Number: EC 3.1.15.-

Grant and Affiliation Information for Substrate specificity of nuclease TT1 from Thermus thermophilus HB8.

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Country: JAPAN

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MEDLINETA: J Biochem

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