Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells.

Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Research Abstract Details 

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Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Abstract Text:

Elena V Leonova,Keith E Pennington,Paul H Krebsbach,David H Kohn,

Understanding the mechanisms of substrate based control of cell function is critical to the design of biomaterials. Cells interact with their extracellular matrix through cell adhesion contacts. We have previously described the self assembly of bone-like mineral onto an organic template and have shown that these biomimetic surfaces lead to an increased volume fraction of bone regenerated in vivo. In the present study, we compared the distribution of cell adhesion contacts, cell spreading, and cell motility of murine bone marrow stromal cells (BMSC) on mineralized vs. nonmineralized substrates. We developed a new approach for quantification of cell-material interactions and demonstrated that cell adhesion contacts on mineralized substrates were distributed throughout the cell surface contacting the substrate, whereas on nonmineralized substrates cell adhesion contacts were present near the cell periphery. We propose that mineralized substrates stimulate the predominant expression of fibrillar contacts, and nonmineralized substrates stimulate expression of focal adhesion contacts. Cell motility assays with colloidal gold demonstrated a statistically significant decrease in the average phagokinetic index of migrating cells on mineralized vs. nonmineralized substrates after 90 min of cell seeding. We propose that the physical-chemical properties of the substrate, altered by mineralization, cause expression of specific types of cell contacts and, as a result, modify molecular mechanisms responsible for cell spreading, motility, and possibly differentiation.

Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Publishing Authors By Initials

EV Leonova,KE Pennington,PH Krebsbach,DH Kohn,

For similar cells: stem cells research abstracts see: cells: stem cells research

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Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biomedical materials research. Part A

VOLUME: 79

Page Numbers: 263-70

Journal Abbreviation:

ISSN: 1549-3296

DAY: 3

MONTH: Nov

YEAR: 2006

Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: cytology

Chemical & Substance for Abstract: Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells. Information

Substance Name: Lactic Acid

Registry Number: 50-21-5

Grant and Affiliation Information for Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells.

AFFILIATION: Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, 48109-1078, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: R01 DE 015411

ACRONYM: DE

MEDLINETA: J Biomed Mater Res A

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