Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa.

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Abstract Text:

Jamal I Saada,Irina V Pinchuk,Carlos A Barrera,Patrick A Adegboyega,Giovanni Suarez,Randy C Mifflin,John F Di Mari,Victor E Reyes,Don W Powell,

The human gastrointestinal mucosa is exposed to a diverse normal microflora and dietary Ags and is a common site of entry for pathogens. The mucosal immune system must respond to these diverse signals with either the initiation of immunity or tolerance. APCs are important accessory cells that modulate T cell responses which initiate and maintain adaptive immunity. The ability of APCs to communicate with CD4+ T cells is largely dependent on the expression of class II MHC molecules by the APCs. Using immunohistochemistry, confocal microscopy, and flow cytometry, we demonstrate that alpha-smooth muscle actin(+), CD90+ subepithelial myofibroblasts (stromal cells) constitutively express class II MHC molecules in normal colonic mucosa and that they are distinct from professional APCs such as macrophages and dendritic cells. Primary isolates of human colonic myofibroblasts (CMFs) cultured in vitro were able to stimulate allogeneic CD4+ T cell proliferation. This process was dependent on class II MHC and CD80/86 costimulatory molecule expression by the myofibroblasts. We also demonstrate that CMFs, engineered to express a specific DR4 allele, can process and present human serum albumin to a human serum albumin-specific and DR4 allele-restricted T cell hybridoma. These studies characterize a novel cell phenotype which, due to its strategic location and class II MHC expression, may be involved in capture of Ags that cross the epithelial barrier and present them to lamina propria CD4+ T cells. Thus, human CMFs may be important in regulating local immunity in the colon.

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Publishing Authors By Initials

JI Saada,IV Pinchuk,CA Barrera,PA Adegboyega,G Suarez,RC Mifflin,JF Di Mari,VE Reyes,DW Powell,

For similar cells: connective tissue cells: stromal cells research abstracts see: cells: connective tissue cells: stromal cells research

PUBMED ID PMID:

MEDLINE DATE:

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 177

Page Numbers: 5968-79

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 1

MONTH: Nov

YEAR: 2006

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Keywords Mesh Terms:

KEYWORDS: Stromal Cells

MESH TERMS: immunology

Chemical & Substance for Abstract: Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa. Information

Substance Name: Interferon Type II

Registry Number: 82115-62-6

Grant and Affiliation Information for Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa.

AFFILIATION: Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK 56338

ACRONYM: DK

MEDLINETA: J Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa Related Publications

• A Comparison of Static and Dynamic Arch Measurements in High and Low Arched Females in Two Dynamic Movements: 1878: Board #166 10:30 AM - 10:30 AM.
• A role for mammalian target of rapamycin in regulating T cell activation versus anergy.
• Agonist and antagonist effects of 15R-prostaglandin (PG) D2 and 11-methylene-PGD2 on human eosinophils and basophils.
• Airway epithelial cells synthesize the lipid mediator 5-oxo-ETE in response to oxidative stress.
• Co-culture of ovine eggs with oviductal cells and trophoblastic vesicles.
• Cutting Edge: TCR-induced NAB2 enhances T cell function by coactivating IL-2 transcription.
• Cynical hostility and carotid atherosclerosis in African American and white women: the Study of Women's Health Across the Nation (SWAN) Heart Study.
• Cytoreductive surgery for gynecologic malignancies-new standards of care.
• Developing a partnership model for cancer screening with community-based organizations: the ACCESS breast cancer education and outreach project.
• Developmental differences in elimination of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) during Xenopus laevis development.
• Differential methylation hybridization array of endometrial cancers reveals two novel cancer-specific methylation markers.
• Discrimination of dairy industry isolates of the Lactobacillus casei group.
• Effects of dietary vitamin E on the biosynthesis of 5-lipoxygenase products by rat polymorphonuclear leukocytes (PMNL).
• Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities.
• Group therapy in a detoxification unit.
• Inhibition of phosphatidylinositol-3-kinase and mitogen-activated protein kinase kinase 1/2 prevents melanoma development and promotes melanoma regression in the transgenic TPRas mouse model.
• Learning-induced glutamate receptor phosphorylation resembles that induced by long term potentiation.
• Levels of medical care in the global war on terrorism.
• Match and Motion Analyses of High Loading Movements in NCAA Men's Basketball Competitions: 934: June 1 5:00 PM - 5:15 PM.
• Metabolism of 5-hydroxy-6,8,11,14-eicosatetraenoic acid by human endothelial cells.
• Physical Activity and Intraabdominal Fat in a Biracial Sample of Women at Midlife: 2135: Board #48 June 1 9:00 AM - 10:30 AM.
• Re: Hugh Cairns and the origin of British neurosurgery.
• Regulation of 5-hydroxyeicosanoid dehydrogenase activity in monocytic cells.
• Stress reactions and perceived difficulties of lay responders to a medical emergency.
• Subepithelial myofibroblasts are novel nonprofessional APCs in the human colonic mucosa.
• Surgical orthognathic management of sleep apnea.
• The Simulator - Has it Had its Day? A Study of Radiotherapy Verification.
• The clinical relevance of compound heterozygosity for the C282Y and H63D substitutions in hemochromatosis.
• The role of BMP-2 and BMP-4 in follicle initiation and the murine hair cycle.
• Update in lung cancer 2006.