Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy.

Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Research Abstract Details 

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Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Abstract Text:

Chenbo Zeng,Suwanna Vangveravong,Jinbin Xu,Katherine C Chang,Richard S Hotchkiss,Kenneth T Wheeler,Duanwen Shen,Zhi-Ping Zhuang,Hank F Kung,Robert H Mach,

Sigma-2 receptor agonists have been shown to induce cell death via caspase-dependent and caspase-independent pathways. Unfortunately, there is little information regarding the molecular function of sigma-2 receptors that can explain these results. In this study, two fluorescent probes, SW107 and K05-138, were used to study the subcellular localization of sigma-2 receptors by two-photon and confocal microscopy. The results indicate that sigma-2 receptors colocalize with fluorescent markers of mitochondria, lysosomes, endoplasmic reticulum, and the plasma membrane in both EMT-6 mouse and MDA-MB-435 human breast cancer cells. The fluorescent probe, K05-138, was internalized rapidly, reaching a plateau of fluorescent intensity at 5 min. The internalization of K05-138 was reduced approximately 40% by phenylarsine oxide, an inhibitor of endocytosis. These data suggest that sigma-2 ligands are internalized, in part, by an endocytotic pathway. The localization of sigma-2 receptors in several organelles known to have a role in both caspase-dependent and caspase-independent pathways of cell death supports the conclusions of previous studies suggesting that sigma-2 receptor ligands should be evaluated as potential cancer chemotherapeutic agents.

Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Publishing Authors By Initials

C Zeng,S Vangveravong,J Xu,KC Chang,RS Hotchkiss,KT Wheeler,D Shen,ZP Zhuang,HF Kung,RH Mach,

For similar proteins: membrane proteins: receptors, cell surface: receptors, g-protein-coupled: receptors, opioid: receptors, sigma research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, g-protein-coupled: receptors, opioid: receptors, sigma research

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Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cancer research

VOLUME: 67

Page Numbers: 6708-16

Journal Abbreviation: Cancer Res.

ISSN: 0008-5472

DAY: 15

MONTH: Jul

YEAR: 2007

Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2984705

Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Keywords Mesh Terms:

KEYWORDS: Receptors, sigma

MESH TERMS: chemistry

Chemical & Substance for Abstract: Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy. Information

Substance Name: Caspases

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Subcellular localization of sigma-2 receptors in breast cancer cells using two-photon and confocal microscopy.

AFFILIATION: Department of Radiology, Division of Radiological Sciences, Washington University School of Medicine, 510 S. Kingshighway Boulevard, St. Louis, MO 63110, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA 102869

ACRONYM: CA

MEDLINETA: Cancer Res

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