[Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus]

[Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus] Research Abstract Details 

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[Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus] Abstract Text:

Hong Liu,Sui Cao,Xiu-Bao Ren,Peng Zhang,Xiu-Mei An,Xi-Shan Hao,

In order to find a method suitable for purifying large amount of CD34(+) cells, from 5 cases who accepted autologous peripheral blood stem cell transplantation, CD34(+) cells were collected and enriched by using Isolex 300i (Nexell). Phenotypes were detected by flow cytometry and the biological viability were assayed by the colony-forming experiments and cell expansion experiment in vitro. The results showed that the number of mononuclear cells first collected was about (3.5 - 6.0) x 10(10) and (0.55 - 1.2)% of cells were CD34 positive. The number of positive production was about (2.0 - 3.0) x 10(8); the CD34(+) cells purity was (75 - 85)% and the yield was (40 - 65)%. The CD34(+) cells of positive production could expand up to 2 - 3 times when cultured with SCF + IL3 + FL + TPO + EPO in vitro. The results of colony-forming experiments demonstrated that the CD34(+) cells collected has enough colony-forming ability. All results showed the enriched CD34(+) cells with biological viability. In conclusion, the CD34(+) immunomagnetic isolation apparatus Isolex300i is suitable to clinical application for a large amount of CD34(+) cell enrichment.

[Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus] Publishing Authors By Initials

H Liu,S Cao,XB Ren,P Zhang,XM An,XS Hao,

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[Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing

VOLUME: 12

Page Numbers: 798-802

Journal Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za

ISSN: 1009-2137

DAY: 5

MONTH: Dec

YEAR: 2004

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LANGUAGE: chi

NlmUniqueID: 101084424

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AFFILIATION: Department of Immunology, Affiliated Hospital of Oncology, Tianjing Medical University, Tianjing 300060, China. abcd-lh@eyou.com

Country: China

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MEDLINETA: Zhongguo Shi Yan Xue Ye Xue Za

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