Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages.

Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Research Abstract Details 

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Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Abstract Text:

T Taki,K Takagi,R Kamada,M Matsumoto,K Kojima,

The presence of asialogangliosides on membrane surfaces of rat bone marrow cells and macrophages was studied by an immunological method using anti-glycolipid antibodies with high specificity. The antibodies against N-acetylgalactosaminyl(beta 1-4)galactosyl(beta 1-4)glucosylceramide (GA2), galactosyl(beta 1-3)N-acetylgalactosaminyl(beta 1-4)galactosyl(beta 1-4)glucosylceramide (GA1) and fucosyl(alpha 1-2)galactosyl(beta 1-3)N-acetylgalactosaminyl(beta 1-4)galactosyl(beta 1-4)glucosylceramide (Fuco-GA1) were prepared, and used for cytotoxicity testing and indirect staining by the immunofluorescence technique. In the cytotoxicity test, approximately 40-50% of rat intraperitoneal macrophages were killed by each of the anti-glycolipid antisera. This finding was confirmed by the indirect immunofluorescence staining method with the purified anti-GA2, anti-GA1, and also anti-Fuco-GA1 IgG. When a rat was preimmunized with bovine serum albumin, all macrophages induced in ascites fluid were found to possess GA1 by the staining method. Immature granulocytes were stained specifically with the anti-GA2 antibody. Granulocytes, myelocytes, and megakaryocytes were stained with the anti-GA1 antibody. Blastic myelocytes were stained with the anti-Fuco-GA1 IgG, but lymphocytes were not stained with any of these anti-asialoganglioside antibodies. From these observations, free-type cells such as macrophages and bone marrow cells were found to possess asialogangliosides as membrane components.

Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Publishing Authors By Initials

T Taki,K Takagi,R Kamada,M Matsumoto,K Kojima,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 90

Page Numbers: 1653-60

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1981

Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: analysis

Chemical & Substance for Abstract: Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages. Information

Substance Name: Glycosphingolipids

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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