Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin.

Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Research Abstract Details 

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Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Abstract Text:

H Oshima,T Abe,K Takahashi,

1. When thermolysin was treated with a 100-fold molar excess of 2,4,6-trinitrobenzene-1-sulfonate at pH 8.0 and 37 degrees for 7 h, all 12 amino groups in the enzyme were almost completely trinitrophenylated. The fully trinitrophenylated enzyme still retained more than 80% of its original activity. The amino groups are therefore not essential for activity. 2. When treated with a 100- to 1,000-fold molar excess of N-acetylimidazole at pH 6.5 and 23 degrees for 2 h, thermolysin lost about 54% of its activity with concomitant acetylation of 21 tyrosine residues out of the total of 28 residues. The reaction did not easily proceed any further. This partially inactivated enzyme regained almost full activity upon treatment with hydroxylamine. These modified tyrosine residues are therefore not directly involved in the active site. 3. Thermolysin was not inactivated by treatment with about 100- to 150-fold molar excess of 2-hydroxy-5-nitrobenzyl bromide or dimethyl(2-hydroxy-5-nitrobenzyl)sulfonium bromide at pH 6.0 and room temperature for 1 h both in the presence and absence of 8 M urea. Thus the three tryptophan residues in the enzyme are not accessible to these reagents. When treated with a 4.3- to 43-fold molar excess of N-bromosuccinimide over tryptophan, the enzyme was inactivated to varying extents, depending on the reaction conditions used. In this case, the tyrosine residues appeared to be the most rapidly modified, but tryptophan and histidine residues were also modified with extensive inactivation at higher concentrations of the reagent. The presence of 8 M urea retarded the inactivation.

Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Publishing Authors By Initials

H Oshima,T Abe,K Takahashi,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 65-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1977

Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin. Information

Substance Name: Thermolysin

Registry Number: EC 3.4.24.27

Grant and Affiliation Information for Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin.

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Country: JAPAN

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MEDLINETA: J Biochem

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