Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes.

Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Research Abstract Details 

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Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Abstract Text:

S Yamamoto,S Hase,H Yamauchi,T Tanimoto,T Ikenaka,

Sugar chains of interferon-gamma (IFN-gamma) from human peripheral-blood lymphocytes (PBL) were liberated by hydrazinolysis. After N-acetylation, the reducing end residues of the sugar chains were tagged with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and reversed-phase HPLC. Five major PA-sugar chains were obtained. The structure of each PA-sugar chain was estimated by comparing its elution times on anion exchange, reversed-phase, and size-fractionation HPLC with those of PA-sugar chains of IFN-gamma from the human myelomonocyte cell line HBL-38 (Yamamoto, S. et al. (1989) J. Biochem. 105, 547-555) as standards, and also by comparison of their elution times after partial desialylation. The results showed that IFN-gamma (PBL) contained mono- and disialo-biantennary structures with 0 or 1 mol of fucose residue, as found for IFN-gamma (HBL-38), but the N-acetylneuraminyl alpha 2-6 linkage was dominant in IFN-gamma (PBL), unlike IFN-gamma (HBL-38), which contains both N-acetylneuraminyl alpha 2-3 and alpha 2-6 linkages.

Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Publishing Authors By Initials

S Yamamoto,S Hase,H Yamauchi,T Tanimoto,T Ikenaka,

For similar organic chemicals: carboxylic acids: acids, acyclic: sugar acids: neuraminic acids: sialic acids research abstracts see: organic chemicals: carboxylic acids: acids, acyclic: sugar acids: neuraminic acids: sialic acids research

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Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 105

Page Numbers: 1034-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1989

Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Keywords Mesh Terms:

KEYWORDS: Sialic Acids

MESH TERMS: analysis

Chemical & Substance for Abstract: Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes. Information

Substance Name: Neuraminidase

Registry Number: EC 3.2.1.18

Grant and Affiliation Information for Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes.

AFFILIATION: Department of Chemistry, Osaka University College of Science.

Country: JAPAN

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MEDLINETA: J Biochem

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