Studies on the serine proteases associated with rat liver chromatin.

Studies on the serine proteases associated with rat liver chromatin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Studies on the serine proteases associated with rat liver chromatin. Abstract Text:

K Tsurugi,K Ogata,

The chromatin fraction of rat liver exhibited proteolytic activity caused by serine proteases, with maximal activity at pH 8 or 10. They were analyzed by affinity labeling with [3H]diisopropylfluorophosphate followed by SDS-polyacrylamide gel electrophoresis, and partially purified by gel filtration through Sepharose 6B after selective extraction from the chromatin fraction. The following results were obtained. 1. The chromatin fraction contained three DFP-binding proteins with molecular weights of 52,000, 25,000, and 15,000 daltons, and they were tentatively designated proteins A, B, and C, respectively. Unlike proteins A and B, protein C reacted with DFP more strongly at pH 10 than at pH 8. A greater part of protein B was present in the nucleoli, while the others were predominantly distributed in extra-nucleolar chromatin. 2. Proteins A and B were extracted from the chromatin fraction by 5 M urea and 0.7 M NaCl, respectively; while protein C was not extractable by either solution. Proteins A and B were further purified by gel filtration through Sepharose 6B. 3. Protein B was a neutral protease with a maximal activity for 3H-labeled ribosomal proteins at pH 8 and showed high specificity for basic proteins, such as histone and ribosomal proteins. Protein A was an alkaline protease with a maximal activity at pH 10 and showed proteolytic activity not only for basic proteins but also for hydrophobic proteins, such as casein and non-histone proteins of chromatin. 4. Protein A was activated at pH 8 by high concentrations of NaCl, suggesting the presence of some inhibitor(s). Protein A was converted to protein C at pH 10, and also at pH 8 with high concentrations of NaCl. Thus, protein A is suggested to be the complex of protein C and unknown inhibitor(s). 5. When the chromatin fraction was incubated at pH 10, non-histone proteins were degraded much faster than at pH 8, although H1 histone was degraded at similar rates at both pHs. These results indicate that the chromatin fraction of rat liver contains at least two kinds of serine proteases, B and C, and that protease B is probably involved in the metabolism of basic protein, especially H1 histone. Protease C, the greater part of which associates with some inhibitors to form protein A, may play its main role in the metabolism of non-histone proteins.

Studies on the serine proteases associated with rat liver chromatin. Publishing Authors By Initials

K Tsurugi,K Ogata,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases research

PUBMED ID PMID:

MEDLINE DATE:

Studies on the serine proteases associated with rat liver chromatin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 92

Page Numbers: 1369-81

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1982

Studies on the serine proteases associated with rat liver chromatin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Studies on the serine proteases associated with rat liver chromatin. Keywords Mesh Terms:

KEYWORDS: Serine Endopeptidases

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Studies on the serine proteases associated with rat liver chromatin. Information

Substance Name: Serine Endopeptidases

Registry Number: EC 3.4.21.-

Grant and Affiliation Information for Studies on the serine proteases associated with rat liver chromatin.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Studies on the serine proteases associated with rat liver chromatin Related Publications

• Androgen receptor stimulates bone sialoprotein (BSP) gene transcription via cAMP response element and activator protein 1/glucocorticoid response elements.
• Biological process of carbohydrate attachment to ovalbumin.
• Complete cDNA sequence of the fourth component of murine complement.
• Constitutive expression of Slp genes in mouse strain B10.WR directed by C4 regulatory sequences.
• Effects of essential medicines on cardiovascular products available for the market in Thailand.
• Effects of protein deficiency on serum albumin-synthesizing activity of rat liver--with special reference to the presence of transcriptional controls.
• Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver.
• Effects of the use of high concentrations of Mg2+ in the preparation of proteins from rat liver ribosomes pretreated with polyvinyl sulfate. Improved methods for preparing ribosomal proteins from ribosomal precursor particles of liver nucleoli.
• Evaluation of cleft lip bony depression of piriform margin and nasal deformity with cone beam computed tomography: "retruded-like" appearance and anteroposterior position of the alar base.
• Evidence for exclusive biosynthesis in vivo of serum albumin by bound polysomes of rat liver.
• Fermi-surface reconstruction without breakdown of Kondo screening at the quantum critical point.
• Hemoglobin biosynthesis in the chicken red blood cells and inhibitory effect of actinomycin D.
• Incorporation of 14C-amino acids into basic proteins of 47S and 32S subunits of rat liver ribosomes by a cell-free system.
• Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo).
• Lack of exchange of ribosomal structural proteins with cell-sap proteins of rat liver in vitro.
• Malignant melanoma in a woman with LEOPARD syndrome: identification of a germline PTPN11 mutation and a somatic BRAF mutation.
• Mice constitutive for sex-limited protein (SLP) expression contain multiple Slp gene sequences.
• Preferential synthesis of arginase by free polysomes from rat liver.
• Purification and properties of acylamino acid-releasing enzyme from rat liver.
• Rat liver nucleolar 29.5S RNA as the precursor of nucleolar 28S RNA.
• Stabilizing effect of ribonuclease inhibitor on structure of polysomes and some properties of four classes of ribosomal particles in rat liver cytoplasm.
• Structure and expression of murine fourth complement component (C4) and sex-limited protein (Slp).
• Studies on SDS-phenol methods for extraction of rat liver nuclear RNA. I. Purity, recovery, and specific radioactivity of pulse labeled nuclear RNA obtained by SDS-phenol extraction under various conditions.
• Studies on SDS-phenol methods for extraction of rat liver nuclear RNA. II. Polyacrylamide gel electrophoresis of nuclear RNA obtained using various conditions for SDS-phenol extraction.
• Studies on creatine kinase of skeletal muscle and brain with special reference to subcellular distribution and isozymes.
• Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver.
• The different effects of N-ethylmaleimide and iodoacetamide on the activity of rat liver 60S subunits for peptide bond elongation.
• Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells.
• [Sudden cardiac death]
• cDNA sequence of a novel sex-limited protein (Slp) from mice constitutive for Slp expression. Sequence comparisons suggest that Slp has no functional role.