[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)]

[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Research Abstract Details 

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[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Abstract Text:

A method is described for the administration of suspended material into the rat lung. The rat is narcotized by CO2/air, the trachea is punctured and the suspension is sucked into the lung by the inhaled air (pertracheal administration, p. t. a.). BaP administered by this method is eliminated from the lung rapidly, while metabolites persist for a longer period. The radioactivity detected in the blood can be attributed predominantly to metabolites, a small fraction is unmetabolized benzo(a)pyrene. The activity of benzo(a)pyrene hydroxylase is induced in the lung after p. t. a. of extracts from airborne particulate matter. The activity increases depending on the dose until a plateau of activity is reached at high doses. Pertracheally administered BaP is bound to DNA of the lung. Pretreatment with extracts p.t.a. enhances the binding. After enzymatic digestion of DNA BaP-nucleoside adducts are detected by HPLC. According to data published in the literature to adducts are supposed to be derived from BaP-phenols and BaP-diolepoxides. It is concluded tht the components of airborne particulates can modify the activity of metabolizing enzymes and increase the rate of formation of adducts in the lung in vivo.

[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Publishing Authors By Initials

For similar respiratory system: trachea research abstracts see: respiratory system: trachea research

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[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zentralblatt für Bakteriologie, Mikrobiologie und

VOLUME: 174

Page Numbers: 260-73

Journal Abbreviation:

ISSN: 0174-3015

DAY: 26

MONTH: Dec

YEAR: 1981

[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Information

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LANGUAGE: ger

NlmUniqueID: 8110036

[Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Keywords Mesh Terms:

KEYWORDS: Trachea

MESH TERMS: metabolism

Chemical & Substance for Abstract: [Studies on the induction by extracts from airborne particulates of benzo(a)pyrene hydroxylase and the binding of benzo(a)pyrene to DNA in the rat lung in vivo after pertracheal administration (author's transl)] Information

Substance Name: Benzopyrene Hydroxylase

Registry Number: EC 1.14.14.-

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Country: GERMANY, WEST

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MEDLINETA: Zentralbl Bakteriol Mikrobiol

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Studies on the induction by extracts from airborne particulates of benzoapyrene hydroxylase and the binding of benzoapyrene to DNA in the rat lung in vivo after pertracheal administration author's transl Related Publications

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