Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization.

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Abstract Text:

M Miyano,K Fukui,F Watanabe,S Takahashi,M Tada,M Kanashiro,Y Miyake,

Two recombinant mutants of porcine kidney D-amino acid oxidase [EC 1.4.3.3, DAO], in which Tyr(228) and His(307) are replaced with Phe and Leu, respectively, have been expressed in Escherichia coli and purified to apparent homogeneity. The molecular size and amino-terminal sequence of the two mutants were the same as those of the native DAO. Kinetic analysis revealed that the Michaelis constants of the Phe-228 and Leu-307 mutants for D-alanine were 71- and 10-fold and the inhibition constants for benzoate, a potent competitive inhibitor, were 1,189- and 18-fold greater than those of the native DAO, respectively. The maximum velocities of the Phe-228 and Leu-307 mutants were 66 and 58% that of the native DAO. The kinetically estimated dissociation constant of the Leu-307 mutant for FAD was 28-fold greater than that of the native DAO, whereas the value of the Phe-228 mutant was comparable to that of the native DAO. The Leu-307 mutant and the recombinant wild-type DAO were inactivated by D-propargylglycine (D-PG), a suicide substrate. However, the Phe-228 mutant was resistant to the inactivation. Absorption peaks of the Phe-228 mutant were blue-shifted about 10 nm from the corresponding peaks of the wild-type DAO, and the oxidized form was fully reduced by D-alanine without appearance of the purple intermediate.(ABSTRACT TRUNCATED AT 250 WORDS)

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Publishing Authors By Initials

M Miyano,K Fukui,F Watanabe,S Takahashi,M Tada,M Kanashiro,Y Miyake,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

PUBMED ID PMID:

MEDLINE DATE:

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 109

Page Numbers: 171-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1991

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization. Information

Substance Name: D-Amino-Acid Oxidase

Registry Number: EC 1.4.3.3

Grant and Affiliation Information for Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization.

AFFILIATION: Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Studies on Phe-228 and Leu-307 recombinant mutants of porcine kidney D-amino acid oxidase: expression, purification, and characterization Related Publications

• A 13C-NMR study on the interaction of riboflavin with egg white riboflavin binding protein.
• A protein associated with Toll-like receptor (TLR) 4 (PRAT4A) is required for TLR-dependent immune responses.
• A spin label study on human low density lipoprotein.
• A spin trap study on anaerobic dehalogenation of halothane by a reconstituted liver microsomal cytochrome P-450 enzyme system.
• Activation of kidney prorenin by kidney cathepsin B isozymes.
• Apoptotic cell clearance and autoimmune disorder.
• Clinical significance of aminopeptidase N in non-small cell lung cancer.
• Community-acquired methicillin-resistant Staphylococcus aureus infection with fatal necrotizing pneumonia from lip abscess: a case report.
• Control of Stripelike and Hexagonal Self-Assembly of Gold Nanoparticles by the Tuning of Interactions between Triphenylene Ligands.
• Electron spin resonance studies on the selective labeling of N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)maleimide of rat liver mitochondria.
• Hemagglutination of preoperative blood donation in patients with rheumatoid arthritis.
• Highly efficient reverse transfection with siRNA in multiple wells of microtiter plates.
• Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via activation of ERK1/2 and JNK pathways.
• Identification of twinfilin-2 as a factor involved in neurite outgrowth by RNAi-based screen.
• Mechanistic studies on the reverse reaction process of D-amino acid oxidase.
• Purification and characterization of prorenin from porcine kidney.
• Purification and characterization of renin binding protein (RnBP) from porcine kidney.
• Purification and characterization or porcine kidney cathepsin B.
• Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin.
• Purification of high molecular weight (HMW) renin from porcine kidney and direct evidence that the HMW renin is a complex of renin with renin binding protein (RnBP).
• Repeated radiofrequency ablation for the distant recurrence in the liver in patients with chronic hepatitis C virus infection achieving long-term survival.
• Risk factors for postpartum hemorrhage after vaginal delivery of twins.
• Serum KL-6 as a novel tumor marker for hepatocellular carcinoma in hepatitis C virus infected patients.
• Some properties of D-amino-acid oxidase apoenzyme from beef kidney.
• Spectroscopic studies on the photoreaction of choline oxidase, a flavoprotein, with covalently bound flavin.
• Spring-bead animation of viscoelastic materials.
• Studies on the reaction mechanism of NADPH-adrenodoxin reductase with NADPH.
• Transcorneal electrical stimulation of retina to treat longstanding retinal artery occlusion.
• Understanding the behavior of invariant NKT cells in autoimmune diseases.
• [Hypertension in pregnant women]