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Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver.

Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Research Abstract Details 

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  • Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Abstract Text:

    k ueharaK Uehara,t tanimotoT Tanimoto,

    D-Erythrulose reductase of beef liver was crystallized from ammonium sulfate solution at pH 8.17. The crystals are needle-shaped. The enzyme protein contains 851 amino acid residues per mole of the enzyme: Lys28, His11, Arg52, Asp79, Thr58, Ser56, Glu68, Pro20, Gly80, Ala107, Val112, Met24, Ile31, Leu88, Tyr7, Phe22, Trp4, and Cys4. The enzyme is inactivated by exposure to temperatures below 12degrees. The inactivation is accelerated by increasing the salt concentration and decreasing the enzyme concentration. The pH of the medium also has a pronounced effect, the maximum stability of the enzyme is obtained at pH 8.5. NADP+ protected the enzyme from cold inactivation at all stages of the process and also afforded protection against inactivation by heat and pH. The cold inactivation of the enzyme is accompanied by dissociation of the enzyme protein to subunits.

    Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Publishing Authors By Initials

    k ueharaK Uehara,t tanimotoT Tanimoto,

    For similar carbohydrates: monosaccharides: tetroses research abstracts see: carbohydrates: monosaccharides: tetroses research

    PUBMED ID PMID:

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    Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 78

    Page Numbers: 519-26

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Sep

    YEAR: 1975

    Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Keywords Mesh Terms:

    KEYWORDS: Tetroses

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver. Information

    Substance Name: Alcohol Oxidoreductases

    Registry Number: EC 1.1.-

    Grant and Affiliation Information for Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver.

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    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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