Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver.

Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Research Abstract Details 

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Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Abstract Text:

K Uehara,S Mannen,S Hosomi,T Miyashita,

D-Erythrulose reductase from chicken liver has been purified to homogeneity as judged by acrylamide gel electrophoresis and ultracentrifugation. The overall purification of the enzyme was 164-fold from a crude extract. The enzyme was crystallized from ammonium sulfate solution at pH 7.0 to give hexagonal plates. The molecular weight determined by sedimentation equilibrium analysis was 94,600 and that by SDS-polyacrylamide gel electrophoresis was 22,400, which suggests a tetrameric structure for the native enzyme. The enzyme was found to contain up to 3 molecules of NADP+ per enzyme; this high amount of NADP+ resulted in a higher absorption at 260 nm than at 280 nm. The extinction coefficient of the enzyme at 290 nm was found to be 4.0. The contents of various amino acids were very similar to those of the beef liver enzyme formerly crystallized in our laboratory. The isoelectric point of the enzyme determined by Ampholine isoelectric focusing was pH 6.43. The enzyme was shown to catalyze the reduction of D-erythrulose to D-threitol with the concomitant oxidation of NAD(P)H to NAD(P)+, and was highly specific to D-erythrulose with an apparent Km of 0.38 mM. NADH was less effective than NADPH and the Km's for NADH and NADPH were 67 micrometers and 7.9 micrometers, respectively. D-Threitol was slightly oxidized by the enzyme with either NADP+ or NAD+ as a cofactor at pH's 7.5 and 9.0.

Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Publishing Authors By Initials

K Uehara,S Mannen,S Hosomi,T Miyashita,

For similar carbohydrates: monosaccharides: tetroses research abstracts see: carbohydrates: monosaccharides: tetroses research

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Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 47-55

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1980

Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Keywords Mesh Terms:

KEYWORDS: Tetroses

MESH TERMS: metabolism

Chemical & Substance for Abstract: Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver. Information

Substance Name: D-erythrulose reductase

Registry Number: EC 1.1.1.162

Grant and Affiliation Information for Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver.

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Country: JAPAN

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MEDLINETA: J Biochem

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