Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT.

Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Research Abstract Details 

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Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Abstract Text:

T Date,K Suzuki,K Imahori,

Two types of RNA polymerases [EC 2.7.7.6], polymerases A and B, exist in thermophilic bacteria, Thermus thermophilus HB8. Polymerase B is apparently like the core enzyme of polymerase A but is active only when an alternating copolymer of deoxyadenylic and deoxythymidylic acids (poly d(A-T)) or a mixture of homopolymers of deoxyadenylic acid and deoxythymidylic acid (poly dAdT) is used as a template. Polymerase B was further characterized to elucidate its relation to polymerase A and to determine why it is inactive on natural DNA's. 1. Polymerase B did not show pyrophosphate exchange activity. Dinucleoside monophosphates did not activate the RNA-synthesizing activity. The results suggested that polymerase B had no initiation and presumably no elongation activities. 2. Polymerase B had about 6 times greater affinity to DNA than polymerase A. The binding of polymerase B to DNA was, however, reversible. The complex of DNA with polymerase A was stable and the polymerase was not removed from the initial complex even when a large amount of DNA was added. 3. E. coli sigma subunit could not stimulate the activity of polymerase B toward DNA's. 4. Polymerase B could utilize poly d(A-T) and poly dAdT as templates, but could not use Bacillus cereus DNA though the structure is reported to be similar to that of poly d(A-T).

Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Publishing Authors By Initials

T Date,K Suzuki,K Imahori,

For similar tissues: lymphoid tissue: thymus gland research abstracts see: tissues: lymphoid tissue: thymus gland research

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Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 78

Page Numbers: 955-67

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1975

Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Keywords Mesh Terms:

KEYWORDS: Thymus Gland

MESH TERMS: metabolism

Chemical & Substance for Abstract: Studies on a thermophilic RNA polymerase which is active only on poly d(A-T) and poly dAdT. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

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Country: JAPAN

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MEDLINETA: J Biochem

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