Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box.

Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. Research Abstract Details 

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Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. Abstract Text:

Induction of nod genes in Rhizobium and Bradyrhizobium species is dependent on the presence of plant-produced flavonoids, the NodD protein, and the cis-acting nod box promoter sequence. Although the nodD (nodD1) gene in Rhizobium species is constitutively expressed, nodD1 expression in Bradyrhizobium japonicum is inducible by isoflavones in a manner similar to that of the nodYABC operon. A consensus nod box sequence is found 5' of the nodYABC operon, whereas a presumptive, nod box-like sequence is found 5' of the nodD1 gene. As an initial step toward examining the nodD1 promoter, the transcriptional start sites of the nodD1 and nodYABC operons were determined and found to be 44 and 28 bp, respectively, downstream of their respective nod box sequences. A series of deletions of the nodD1 promoter were constructed and fused to the lacZ gene. Analysis of the activity of these deletions clearly showed that the divergent nod box sequence was essential for nodD1 induction by isoflavones or soybean seed extract. The induction of nodD1 expression requires NodD1, as tested in B. japonicum and in a heterologous system, Agrobacterium tumefaciens. On the basis of these data, we analyzed the published nod box sequences and propose a new consensus sequence composed of paired 9-bp repeats. Analysis of the nodD1 nod box and synthetic constructs of the nocYABC nod box indicate that at least two 9-bp repeats are required for NodD1-mediated induction. Furthermore, insertions between the paired repeats of the nodYABC nod box suggest that orientation of the repeats on opposite faces of the DNA helix is essential for maximum nod gene expression.

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Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 173

Page Numbers: 3356-65

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 18

MONTH: Jun

YEAR: 1991

Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box.

AFFILIATION: Center for Legume Research, University of Tennessee, Knoxville 37996-0845.

Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: GM40183-03

ACRONYM: GM

MEDLINETA: J Bacteriol

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