Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit.

Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Research Abstract Details 

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Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Abstract Text:

Christine M Dunham,Maria Selmer,Steven S Phelps,Ann C Kelley,Tsutomu Suzuki,Simpson Joseph,V Ramakrishnan,

During translation, some +1 frameshift mRNA sites are decoded by frameshift suppressor tRNAs that contain an extra base in their anticodon loops. Similarly engineered tRNAs have been used to insert nonnatural amino acids into proteins. Here, we report crystal structures of two anticodon stem-loops (ASLs) from tRNAs known to facilitate +1 frameshifting bound to the 30S ribosomal subunit with their cognate mRNAs. ASL(CCCG) and ASL(ACCC) (5'-3' nomenclature) form unpredicted anticodon-codon interactions where the anticodon base 34 at the wobble position contacts either the fourth codon base or the third and fourth codon bases. In addition, we report the structure of ASL(ACGA) bound to the 30S ribosomal subunit with its cognate mRNA. The tRNA containing this ASL was previously shown to be unable to facilitate +1 frameshifting in competition with normal tRNAs (Hohsaka et al. 2001), and interestingly, it displays a normal anticodon-codon interaction. These structures show that the expanded anticodon loop of +1 frameshift promoting tRNAs are flexible enough to adopt conformations that allow three bases of the anticodon to span four bases of the mRNA. Therefore it appears that normal triplet pairing is not an absolute constraint of the decoding center.

Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Publishing Authors By Initials

CM Dunham,M Selmer,SS Phelps,AC Kelley,T Suzuki,S Joseph,V Ramakrishnan,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus: thermus thermophilus research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus: thermus thermophilus research

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Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 817-23

Journal Abbreviation: RNA

ISSN: 1355-8382

DAY: 6

MONTH: 04

YEAR: 2007

Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Information

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LANGUAGE: eng

NlmUniqueID: 9509184

Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Keywords Mesh Terms:

KEYWORDS: Thermus thermophilus

MESH TERMS: metabolism

Chemical & Substance for Abstract: Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit. Information

Substance Name: RNA, Transfer

Registry Number: 9014-25-9

Grant and Affiliation Information for Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit.

AFFILIATION: MRC-Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom.

Country: United States

AGENCY: United States NIGMS

GRANT: GM67624

ACRONYM: GM

MEDLINETA: RNA

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