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Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator.

Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator. Research Abstract Details 

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  • Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator. Abstract Text:

    In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.

    Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator. Publishing Authors By Initials

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    MEDLINE DATE:

    Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Optics letters

    VOLUME: 33

    Page Numbers: 1629-31

    Journal Abbreviation:

    ISSN: 0146-9592

    DAY: 15

    MONTH: Jul

    YEAR: 2008

    Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 7708433

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    Grant and Affiliation Information for Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator.

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    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Opt Lett

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