Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics.

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Abstract Text:

Andrew J Antczak,Toshiaki Tsubota,Paul D Kaufman,James M Berger,

BACKGROUND: The histone H3/H4 chaperone Asf1 (anti-silencing function 1) is required for the establishment and maintenance of proper chromatin structure, as well as for genome stability in eukaryotes. Asf1 participates in both DNA replication-coupled (RC) and replication-independent (RI) histone deposition reactions in vitro and interacts with complexes responsible for both pathways in vivo. Asf1 is known to directly bind histone H3, however, high-resolution structural information about the geometry of this interaction was previously unknown. RESULTS: Here we report the structure of a histone/histone chaperone interaction. We have solved the 2.2 A crystal structure of the conserved N-terminal immunoglobulin fold domain of yeast Asf1 (residues 2-155) bound to the C-terminal helix of yeast histone H3 (residues 121-134). The structure defines a histone-binding patch on Asf1 consisting of both conserved and yeast-specific residues; mutation of these residues abrogates H3/H4 binding affinity. The geometry of the interaction indicates that Asf1 binds to histones H3/H4 in a manner that likely blocks sterically the H3/H3 interface of the nucleosomal four-helix bundle. CONCLUSION: These data clarify how Asf1 regulates histone stoichiometry to modulate epigenetic inheritance. The structure further suggests a physical model in which Asf1 contributes to interpretation of a "histone H3 barcode" for sorting H3 isoforms into different deposition pathways.

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Publishing Authors By Initials

AJ Antczak,T Tsubota,PD Kaufman,JM Berger,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

PUBMED ID PMID:

MEDLINE DATE:

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: BMC structural biology

VOLUME: 6

Page Numbers: 26

Journal Abbreviation: BMC Struct. Biol.

ISSN: 1472-6807

DAY: 13

MONTH: 12

YEAR: 2006

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101088689

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: genetics

Chemical & Substance for Abstract: Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. Information

Substance Name: Silent Information Regulator Proteins, S

Registry Number: 0

Grant and Affiliation Information for Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics.

AFFILIATION: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA. antczaka@berkeley.edu <antczaka@berkeley.edu>

Country: England

AGENCY: United States NCI

GRANT: CA077373

ACRONYM: CA

MEDLINETA: BMC Struct Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: 2IDC

Number Hits: 0

Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics Related Publications

• A cranial base of Australopithecus robustus from the hanging remnant of Swartkrans, South Africa.
• Anisomycin disrupts a contextual memory following reactivation in a cocaine-induced locomotor activity paradigm.
• Carnivore repatriation and holarctic prey: narrowing the deficit in ecological effectiveness.
• Change in prevalence of psychiatric disorders from ages 21 to 30 in a community sample.
• Competition between split and nonmanipulated embryos in the production of identical piglets.
• Connecting the dots: an invariant migration corridor links the Holocene to the present.
• DNA replication initiation: mechanisms and regulation in bacteria.
• Development of a zona-free hamster ova bioassay for goat sperm.
• FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies.
• H2B ubiquitylation acts as a barrier to Ctk1 nucleosomal recruitment prior to removal by Ubp8 within a SAGA-related complex.
• Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI.
• Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor.
• Importation of Acinetobacter baumannii into a burn unit: a recurrent outbreak of infection associated with widespread environmental contamination.
• Large genomic fibrillin-1 (FBN1) gene deletions provide evidence for true haploinsufficiency in Marfan syndrome.
• Maximizing operating room and recovery room capacity in an era of constrained resources.
• Molecular assays for monitoring HIV infection and antiretroviral therapy.
• Nonlinear dynamic model of CA1 short-term plasticity using random impulse train stimulation.
• Organisational determinants of the use of physical restraints: a multilevel approach.
• Parthenolide inhibits IkappaB kinase, NF-kappaB activation, and inflammatory response in cystic fibrosis cells and mice.
• Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm.
• Racial disparities in colorectal cancer.
• Relationship of IgG and IgM autoantibodies to oxidized low density lipoprotein with coronary artery disease and cardiovascular events.
• Residue-specific NH exchange rates studied by NMR diffusion experiments.
• Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin.
• Spin precession and avalanches.
• Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics.
• The association between caregiver burden and sleep disturbances in partners of patients with Parkinson's disease.
• The other children: a survey of child abuse physicians on the medical evaluation of children living with a physically abused child.
• Willingness to pay versus willingness to buy: what defines value in healthcare?
• [Primary metastatic renal metastatic carcinoid tumor in a young male patient: a contribution to the differential diagnosis of neuroendocrine tumors]