Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway.

Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway. Research Abstract Details 

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Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway. Abstract Text:

Kexin Huang,Ian Ferrin-O'Connell,Wei Zhang,Gordon A Leonard,Erin K O'Shea,Florante A Quiocho,Kexin Huang,Ian Ferrin-O'Connell,Wei Zhang,Gordon A Leonard,Erin K O'Shea,Florante A Quiocho,Kexin Huang,Ian Ferrin-O'Connell,Wei Zhang,Gordon A Leonard,Erin K O'Shea,Florante A Quiocho,

The ability to sense and respond appropriately to environmental changes is a primary requirement of all living organisms. In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes involved in the regulation of phosphate acquisition from the ambient environment. A signal transduction pathway (the PHO pathway) mediates this response, with Pho85-Pho80 playing a vital role. Here we report the X-ray structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex functioning in transcriptional regulation in response to environmental changes. The structure revealed a specific salt link between a Pho85 arginine and a Pho80 aspartate that makes phosphorylation of the Pho85 activation loop dispensable and that maintains a Pho80 loop conformation for possible substrate recognition. It further showed two sites on the Pho80 cyclin for high-affinity binding of the transcription factor substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant to each other and the active site.

Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway. Publishing Authors By Initials

K Huang,I Ferrin-O'Connell,W Zhang,GA Leonard,EK O'Shea,FA Quiocho,K Huang,I Ferrin-O'Connell,W Zhang,GA Leonard,EK O'Shea,FA Quiocho,K Huang,I Ferrin-O'Connell,W Zhang,GA Leonard,EK O'Shea,FA Quiocho,

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Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular cell

VOLUME: 28

Page Numbers: 614-23

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 30

MONTH: Nov

YEAR: 2007

Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway. Information

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LANGUAGE: eng

NlmUniqueID: 9802571

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Grant and Affiliation Information for Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway.

AFFILIATION: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Country: United States

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MEDLINETA: Mol Cell

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