Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs.

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Abstract Text:

Kirsten Mattison,J Scott Wilbur,Magdalene So,Richard G Brennan,

Neisseria gonorrhoeae is a sexually transmitted pathogen that initiates infections in humans by adhering to the mucosal epithelium of the urogenital tract. The bacterium then enters the apical region of the cell and traffics across the cell to exit into the subepithelial matrix. Mutations in the fast intracellular trafficking (fitAB) locus cause the bacteria to transit a polarized epithelial monolayer more quickly than the wild-type parent and to replicate within cells at an accelerated rate. Here, we describe the crystal structure of the toxin-antitoxin heterodimer, FitAB, bound to a high affinity 36-bp DNA fragment from the fitAB promoter. FitA, the antitoxin, binds DNA through its ribbon-helix-helix motif and is tethered to FitB, the toxin, to form a heterodimer by the insertion of a four turn alpha-helix into an extensive FitB hydrophobic pocket. FitB is composed of a PIN (PilT N terminus) domain, with a central, twisted, 5-stranded parallel beta-sheet that is open on one side and flanked by five alpha-helices. FitB in the context of the FitAB complex does not display nuclease activity against tested PIN substrates. The FitAB complex points to the mechanism by which antitoxins with RHH motifs can block the activity of toxins with PIN domains. Interactions between two FitB molecules result in the formation of a tetramer of FitAB heterodimers, which binds to the 36-bp DNA fragment and provides an explanation for how FitB enhances the DNA binding affinity of FitA.

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Publishing Authors By Initials

K Mattison,JS Wilbur,M So,RG Brennan,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research

PUBMED ID PMID:

MEDLINE DATE:

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 37942-51

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 18

MONTH: 09

YEAR: 2006

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Amino Acid

MESH TERMS: metabolism

Chemical & Substance for Abstract: Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs.

AFFILIATION: Department of Biochemistry, Oregon Health and Science University, Portland, Oregon 97239, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI47260

ACRONYM: AI

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs Related Publications

• 278.
• A phase I pilot study of autologous heat shock protein vaccine HSPPC-96 in patients with resected pancreatic adenocarcinoma.
• Adolescent psychosocial risk factors for severe intimate partner violence in young adulthood.
• BK virus nephropathy and kidney transplantation.
• Cognitive performance and age: norms from the Maine-Syracuse Study.
• Cross-cultural factors necessary to enable design of flexible consumer health informatics systems (CHIS).
• Designing study nurses' training to enhance research integrity: a macroergonomic approach.
• Development of lung cancer before the age of 50: the role of xenobiotic metabolizing genes.
• Does solar exposure, as indicated by the non-melanoma skin cancers, protect from solid cancers: vitamin D as a possible explanation.
• Effect of an evidence-based education programme on ED discharge advice for febrile children.
• Evidence for two schizophrenia susceptibility genes on chromosome 22q13.
• Fibrinogen Columbus: a novel gamma Gly200Val mutation causing hypofibrinogenemia in a family with associated thrombophilia.
• Folate-related genes and the risk of tobacco-related cancers in Central Europe.
• Incidence, predictors, and associated outcomes of atrial fibrillation after kidney transplantation.
• Influence of increasing dietary intake on whole body protein kinetics in normal man.
• Lactate concentrations in incisions indicate ischemic-like conditions may contribute to postoperative pain.
• Letters to the editor.
• Multidrug-binding transcription factor QacR binds the bivalent aromatic diamidines DB75 and DB359 in multiple positions.
• Nerve growth factor expression after plantar incision in the rat.
• New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene.
• Palladium asymmetric allylic alkylation of prochiral nucleophiles: horsfiline.
• Second primary cancers in patients with nasopharyngeal carcinoma: a pooled analysis of 13 cancer registries.
• Strain and sex influence on pain sensitivity after plantar incision in the mouse.
• Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs.
• The association of sequence variants in DNA repair and cell cycle genes with cancers of the upper aerodigestive tract.
• The glycosyltransferases of Mycobacterium tuberculosis - roles in the synthesis of arabinogalactan, lipoarabinomannan, and other glycoconjugates.
• Thigh muscle stiffness assessed with magnetic resonance elastography in hyperthyroid patients before and after medical treatment.
• Tuberculosis: diagnostics.
• Understanding the sex difference in vulnerability to adolescent depression: an examination of child and parent characteristics.
• Unexplained rash in a female college basketball player: 1019: may 30 3:35 PM - 3:55 PM.