Structure of Cdc42 bound to the GTPase binding domain of PAK.

Structure of Cdc42 bound to the GTPase binding domain of PAK. Research Abstract Details 

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Structure of Cdc42 bound to the GTPase binding domain of PAK. Abstract Text:

A Morreale,M Venkatesan,H R Mott,D Owen,D Nietlispach,P N Lowe,E D Laue,

The Rho family GTPases, Cdc42, Rac and Rho, regulate signal transduction pathways via interactions with downstream effector proteins. We report here the solution structure of Cdc42 bound to the GTPase binding domain of alphaPAK, an effector of both Cdc42 and Rac. The structure is compared with those of Cdc42 bound to similar fragments of ACK and WASP, two effector proteins that bind only to Cdc42. The N-termini of all three effector fragments bind in an extended conformation to strand beta2 of Cdc42, and contact helices alpha1 and alpha5. The remaining residues bind to switches I and II of Cdc42, but in a significantly different manner. The structure, together with mutagenesis data, suggests reasons for the specificity of these interactions and provides insight into the mechanism of PAK activation.

Structure of Cdc42 bound to the GTPase binding domain of PAK. Publishing Authors By Initials

A Morreale,M Venkatesan,HR Mott,D Owen,D Nietlispach,PN Lowe,ED Laue,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins: rac gtp-binding proteins research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins: rac gtp-binding proteins research

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Structure of Cdc42 bound to the GTPase binding domain of PAK. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature structural biology

VOLUME: 7

Page Numbers: 384-8

Journal Abbreviation: Nat. Struct. Biol.

ISSN: 1072-8368

DAY: 19

MONTH: May

YEAR: 2000

Structure of Cdc42 bound to the GTPase binding domain of PAK. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421566

Structure of Cdc42 bound to the GTPase binding domain of PAK. Keywords Mesh Terms:

KEYWORDS: rac GTP-Binding Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Structure of Cdc42 bound to the GTPase binding domain of PAK. Information

Substance Name: rac GTP-Binding Proteins

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Structure of Cdc42 bound to the GTPase binding domain of PAK.

AFFILIATION: Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.

Country: UNITED STATES

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MEDLINETA: Nat Struct Biol

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