Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states.

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Abstract Text:

Laure Yatime,Yves Mechulam,Sylvain Blanquet,Emmanuelle Schmitt,Laure Yatime,Yves Mechulam,Sylvain Blanquet,Emmanuelle Schmitt,

Initiation of translation in eukaryotes and in archaea involves eukaryotic/archaeal initiation factor (e/aIF)1 and the heterotrimeric initiation factor e/aIF2. In its GTP-bound form, e/aIF2 provides the initiation complex with Met-tRNA(i)(Met). After recognition of the start codon by initiator tRNA, e/aIF1 leaves the complex. Finally, e/aIF2, now in a GDP-bound form, loses affinity for Met-tRNA(i)(Met) and dissociates from the ribosome. Here, we report a 3D structure of an aIF2 heterotrimer from the archeon Sulfolobus solfataricus obtained in the presence of GDP. Our report highlights how the two-switch regions involved in formation of the tRNA-binding site on subunit gamma exchange conformational information with alpha and beta. The zinc-binding domain of beta lies close to the guanine nucleotide and directly contacts the switch 1 region. As a result, switch 1 adopts a not yet described conformation. Moreover, unexpectedly for a GDP-bound state, switch 2 has the "ON" conformation. The stability of these conformations is accounted for by a ligand, most probably a phosphate ion, bound near the nucleotide binding site. The structure suggests that this GDP-inorganic phosphate (Pi) bound state of aIF2 may be proficient for tRNA binding. Recently, it has been proposed that dissociation of eIF2 from the initiation complex is closely coupled to that of Pi from eIF2gamma upon start codon recognition. The nucleotide state of aIF2 shown here is indicative of a similar mechanism in archaea. Finally, we consider the possibility that release of Pi takes place after e/aIF2gamma has been informed of e/aIF1 dissociation by e/aIF2beta.

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Publishing Authors By Initials

L Yatime,Y Mechulam,S Blanquet,E Schmitt,L Yatime,Y Mechulam,S Blanquet,E Schmitt,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 18445-50

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 13

MONTH: 11

YEAR: 2007

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states.

AFFILIATION: Laboratoire de Biochimie, Ecole Polytechnique, Centre National de la Recherche Scientifique, F-91128 Palaiseau Cedex, France.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states Related Publications

• ADCS Prevention Instrument Project: ADCS-clinicians' global impression of change scales (ADCS-CGIC), self-rated and study partner-rated versions.
• Assessing vaccine efficacy for the prevention of acute otitis media by pneumococcal vaccination in children: a methodological overview of statistical practice in randomized controlled clinical trials.
• Biomechanical and immunohistochemical analysis of high hydrostatic pressure-treated Achilles tendons.
• Chapter 22: Assuring the quality, safety and efficacy of HPV vaccines: The scientific basis of regulatory expectations pre- and post-licensure.
• Concomitant use of medications with anticholinergic properties and acetylcholinesterase inhibitors: impact on cognitive and physical functioning in Alzheimer disease.
• Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression.
• Disease processes may be reflected by correlations among tissue kallikrein proteases but not with proteolytic factors uPA and PAI-1 in primary ovarian carcinoma.
• Effects of carrageenan and morphine on acute inflammation and pain in Lewis and Fischer rats.
• Effects of porcine bile on survival of Bacillus cereus vegetative cells and Haemolysin BL enterotoxin production in reconstituted human small intestine media.
• Efficacy of posaconazole and amphotericin B in experimental invasive pulmonary aspergillosis in dexamethasone immunosuppressed rats.
• Estimating the onset of cambial activity in Scots pine in northern Finland by means of the heat-sum approach.
• Force plate for measuring the ground reaction forces in small animal locomotion.
• Forelimb and hindlimb forces in walking and galloping primates.
• FoxO transcription factors suppress Myc-driven lymphomagenesis via direct activation of Arf.
• Gynecological cytology: too old to be a pop star but too young to die.
• Hearing aid device that can be worn in the ear with a housing.
• Imatinib impairs the proliferation and function of CD4+CD25+ regulatory T cells in a dose-dependent manner.
• Immunohistochemical expression and distribution of VEGFR-3 in malignant mesothelioma.
• Immunohistochemical expression of VEGF-A and its ligands in non-neoplastic lesions of the breast sampling-assisted by dynamic angiothermography.
• Lymphatic vessel density and epithelial D2-40 immunoreactivity in pre-invasive and invasive lesions of the uterine cervix.
• Methodological issues in the assessment of substance use phenotypes.
• Prediction of lung cancer using volatile biomarkers in breath.
• Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors.
• Regulatory T cells--the renaissance of the suppressor T cells.
• Requirement of subunit co-assembly and ankyrin-G for M-channel localization at the axon initial segment.
• The Myc-evoked DNA damage response accounts for treatment resistance in primary lymphomas in vivo.
• The association of pregnancy and the development of obesity - results of a systematic review and meta-analysis on the natural history of postpartum weight retention.
• The binding of advanced glycation end products to cell surfaces can be measured using bead-reconstituted cellular membrane proteins.
• The evolution of asymmetric genitalia in spiders and insects.
• [Cardiac imaging in clinical electrophysiology]