Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.

Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Research Abstract Details 

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Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Abstract Text:

Christian Schlieker,Wilhelm A Weihofen,Evelyne Frijns,Lisa M Kattenhorn,Rachelle Gaudet,Hidde L Ploegh,

All members of the herpesviridae contain within their large tegument protein a cysteine protease module that displays deubiquitinating activity. We report the crystal structure of the cysteine protease domain of murine cytomegalovirus M48 (M48(USP)) in a complex with a ubiquitin (Ub)-based suicide substrate. M48(USP) adopts a papain-like fold, with the active-site cysteine forming a thioether linkage to the suicide substrate. The Ub core participates in an extensive hydrophobic interaction with an exposed beta hairpin loop of M48(USP). This Ub binding mode contributes to Ub specificity and is distinct from that observed in other deubiquitinating enzymes. Both the arrangement of active-site residues and the architecture of the interface with Ub lead us to classify this domain as the founding member of a previously unknown class of deubiquitinating enzymes.

Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Publishing Authors By Initials

C Schlieker,WA Weihofen,E Frijns,LM Kattenhorn,R Gaudet,HL Ploegh,

For similar proteins: ubiquitins: ubiquitin research abstracts see: proteins: ubiquitins: ubiquitin research

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Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular cell

VOLUME: 25

Page Numbers: 677-87

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 9

MONTH: Mar

YEAR: 2007

Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Keywords Mesh Terms:

KEYWORDS: Ubiquitin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Information

Substance Name: Cysteine Endopeptidases

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.

AFFILIATION: Department of Biology, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR 15301

ACRONYM: RR

MEDLINETA: Mol Cell

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