Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus.

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Abstract Text:

S Yamamoto,N Okujo,T Yoshida,S Matsuura,S Shinoda,

The structure of vibrioferrin, a siderophore from Vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. HPLC of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. However, upon reinjection of each of those isolated compounds, the original chromatographic pattern was obtained, indicating an equilibrium between two compounds in aqueous solution. Consistent with this finding, most of the NMR signals of vibrioferrin were duplicated. The structure was determined as 1-(2-[2-(5-carboxy-5-hydroxy-2-oxo-1-pyrrolidinyl)propionamide]ethyl) citrate, which exists in two epimeric forms resulting from cyclization between an amidic nitrogen of the alanine residue and a keto group of the 2-ketoglutaric acid residue. Transport experiments with 55Fe-labeled vibrioferrin demonstrated the function of vibrioferrin as a siderophore in V. parahaemolyticus. Kinetic studies with mid-log phase cells revealed that the iron uptake system was receptor-mediated, with Km and Vmax values of 67 nM and 54 pmol Fe/mg cell protein/min, respectively. Moreover, iron uptake mediated by vibrioferrin was blocked both by uncouplers and by ATPase inhibitors.

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Publishing Authors By Initials

S Yamamoto,N Okujo,T Yoshida,S Matsuura,S Shinoda,

For similar bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: vibrionaceae: vibrio: vibrio parahaemolyticus research abstracts see: bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: vibrionaceae: vibrio: vibrio parahaemolyticus research

PUBMED ID PMID:

MEDLINE DATE:

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 115

Page Numbers: 868-74

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1994

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Keywords Mesh Terms:

KEYWORDS: Vibrio parahaemolyticus

MESH TERMS: chemistry

Chemical & Substance for Abstract: Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Information

Substance Name: Iron

Registry Number: 7439-89-6

Grant and Affiliation Information for Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus.

AFFILIATION: Faculty of Pharmaceutical Sciences, Okayama University.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus Related Publications

• A case of childhood multiple sclerosis with peripheral neuropathy.
• Alpha-macroglobulin-like plasma inactivator for Vibrio vulnificus metalloprotease.
• Amino acid sequence of a human kappa type Bence-Jones protein. I. Tryptic peptides of protein Ni.
• Amino acid sequence of a human kappa type Bence-Jones protein. II. Chymotryptic peptides and sequence of protein Ni.
• Anti-human placental antigen complex X-P2 (hPAX-P2) anti-serum recognizes C-terminus of huntingtin-associated protein 1A common to 1B as a determinant marker for the stigmoid body.
• Calculation of focal positions in an optical head for parallel data processing with a monolithic four-beam laser diode.
• Commissural mirror-symmetric excitation and reciprocal inhibition between the two superior colliculi and their roles in vertical and horizontal eye movements.
• Comparative structural studies on the light chains of human immunoglobulins. I. Protein Ka with the Inv(3) allotypic marker.
• Detecting vitreomacular adhesions in eyes with asteroid hyalosis with triamcinolone acetonide.
• Differential gene expression and extracellular secretion of the collagenolytic enzymes by the pathogen Vibrio parahaemolyticus.
• Evidence for involvement of cytochrome P-450-linked oxygenase system in the conversion of C21-steroids to delta 16-C19-steroids catalyzed by pig testicular microsomes.
• Inhibitory effect of alpha 2-macroglobulin on Vibrio vulnificus protease.
• Pancreaticoduodenectomy for bile duct carcinoma of a patient undergoing hemodialysis.
• Presynaptic impulses in the abducens nucleus and their relation to postsynaptic potentials in motoneurons during vestibular nystagmus.
• RNAi-mediated knockdown of juvenile hormone acid O-methyltransferase gene causes precocious metamorphosis in the red flour beetle Tribolium castaneum.
• Sex-steroidal regulation of aromatase mRNA expression in adult male rat brain: a quantitative non-radioactive in situ hybridization study.
• Spinal mechanisms of the functional stretch reflex.
• Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus.
• Structures of two polyamine-containing catecholate siderophores from Vibrio fluvialis.
• Studies on genetically different acid phosphatase of human red cells. I. Comparison of general properties.
• THE ROLE OF AMINO GROUPS IN THE FORMATION OF HEMOGLOBIN-HAPTOGLOBIN COMPLEX.
• The predictive power of self-rated health, activities of daily living, and ambulatory activity for cause-specific mortality among the elderly: a three-year follow-up in urban Japan.
• Two modes of cerebellar input to the parietal cortex in the cat.
• Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system.
• Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system.
• [Effective conferences. Application of POS (problem-oriented system)]
• [Recent advances of juvenile hormone research: synthesis, transport, and molecular action]
• [Studies on the synthesis of sulfanilamide derivatives. IV. Synthesis of N1-acetyl-N1-(6-methoxy-4-pyrimidinyl)sulfanilamide.]
• [Surgical treatment of localized pulmonary aspergillosis (author's transl)]
• [Writing historical novels based on medical history]