Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas.

Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Research Abstract Details 

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Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Abstract Text:

Deisy M Guiretti,Paola A Chabay,Pamela Valva,Claudio G Stefanoff, Barros,Elena De Matteo,Ilana Zalcberg Renault,Maria Victoria Preciado,Rocio Hassan,

Epstein-Barr virus (EBV) is implicated in the pathogenesis of several lymphoid and epithelial neoplasms. Latent membrane protein 1 (LMP1) is the major viral oncogene and it is controversial whether tumor LMP1 variants reflect their geographical predominance or are associated with enhanced oncogenic properties. This study aimed to analyze LMP1 molecular variability of 62 EBV+ Hodgkin's lymphomas and 22 non-neoplastic controls from Brazil and Argentina. EBV association was characterized by EBER-ISH, LMP1 immunohistochemistry and PCR assays for EBNA2 and 3C (typing), LMP1 30 bp deletion (del30) and number of 33 bp tandem repeats. LMP1 C-terminal sequencing was performed in 42 cases. EBV1 was the predominant strain in both geographical Hodgkin's lymphoma groups (average 82%). A higher frequency of del30 variant was observed in lymphomas (41/63) than in non-neoplastic controls (6/22) (OR 4.97, CI 95% 1.53-16.79; P = 0.005, chi(2) test). A large number (5-7) of 33 bp repeat units was characteristic of del30 LMP1 variants (P < 0.0001, Fisher's exact test). Sequence analysis showed a similar mutation spectrum to that described worldwide but none of the current classification schemes could be applied completely. A distinct structural pattern was observed in del30 variants, characterized by a large number of 33 bp repeat units and the presence of a 15 bp insertion encoding the JAK3 Box-1a motif (3/15 wt vs. 16/20 del30; P = 0.001, chi(2) test). The results suggest a pathogenic role for LMP1 del30 variants in Hodgkin's lymphoma from South America and point to particular virus-host molecular mechanisms, such as genomic instability in LMP1 carboxy-terminus, leading to enhanced production and selection of these deletion variants.

Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Publishing Authors By Initials

DM Guiretti,PA Chabay,P Valva,CG Stefanoff,MH Barros,E De Matteo,IZ Renault,MV Preciado,R Hassan,

For similar proteins: viral proteins: viral structural proteins: viral envelope proteins: viral matrix proteins research abstracts see: proteins: viral proteins: viral structural proteins: viral envelope proteins: viral matrix proteins research

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Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of medical virology

VOLUME: 79

Page Numbers: 1730-22

Journal Abbreviation: J. Med. Virol.

ISSN: 0146-6615

DAY: 3

MONTH: Nov

YEAR: 2007

Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Information

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LANGUAGE: eng

NlmUniqueID: 7705876

Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Keywords Mesh Terms:

KEYWORDS: Viral Matrix Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas. Information

Substance Name: Viral Matrix Proteins

Registry Number: 0

Grant and Affiliation Information for Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas.

AFFILIATION: Molecular Biology Laboratory, Bone Marrow Transplantation Centre (CEMO), Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil.

Country: United States

AGENCY: United States NCI

GRANT: R01CA082274-04S1

ACRONYM: CA

MEDLINETA: J Med Virol

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