Structural study on the carbohydrate moiety of human placental alkaline phosphatase.

Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Research Abstract Details 

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Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Abstract Text:

T Endo,H Ohbayashi,Y Hayashi,Y Ikehara,N Kochibe,A Kobata,

Alkaline phosphatase purified from human placenta contains a single asparagine-linked sugar chain in one molecule. The sugar chain was quantitatively liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and NaB3H4 reduction, and separated by paper electrophoresis into one neutral and two acidic fractions. By a combination of sequential exoglycosidase digestion and methylation analysis, the structures of oligosaccharides in the neutral fraction were confirmed to be as follows: Gal beta 1----4GlcNAc beta 1----2Man alpha 1----6(Gal beta 1----4GlcNAc beta 1----2Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(+/- Fuc alpha 1----6)GlcNAc. The acidic oligosaccharide fractions were mixtures of mono- and disialyl derivatives of the neutral fraction. All the sialic acid residues of the sugar chains occur as the NeuAc alpha 2----3Gal group. In the case of monosialyl derivatives, the N-acetylneuraminic acid was exclusively linked to the Man alpha 1----3 arm.

Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Publishing Authors By Initials

T Endo,H Ohbayashi,Y Hayashi,Y Ikehara,N Kochibe,A Kobata,

For similar reproductive and urinary physiology: reproduction: pregnancy research abstracts see: reproductive and urinary physiology: reproduction: pregnancy research

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Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 182-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1988

Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Keywords Mesh Terms:

KEYWORDS: Pregnancy

MESH TERMS: enzymology

Chemical & Substance for Abstract: Structural study on the carbohydrate moiety of human placental alkaline phosphatase. Information

Substance Name: Glycoside Hydrolases

Registry Number: EC 3.2.1.-

Grant and Affiliation Information for Structural study on the carbohydrate moiety of human placental alkaline phosphatase.

AFFILIATION: Department of Biochemistry, University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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