Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane.

Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Research Abstract Details 

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Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Abstract Text:

T Kuroiwa,M Sakaguchi,K Mihara,T Omura,

Co-translational translocation of proteins across the membrane of rough endoplasmic reticulum (ER) is interrupted by particular amino acid sequences, which are functionally termed "stop-transfer sequence." We analyzed the structural requirements for the interruption of the peptide translocation. By the manipulation of the cDNA of interleukin 2 (IL2), which passes through ER membrane co-translationally, the middle portion of the IL2 molecule was replaced with systematically altered hydrophobic segments, leucine, alanine, or leucine/alanine mixed clusters. Furthermore, charged amino acid residues were introduced just downstream of the hydrophobic segments. These modified IL2 peptides were synthesized with wheat germ cell-free system in the presence of rough microsomes and the topology of the peptides in the microsomes was assessed by post-translational digestion with proteinase K. We obtained the following results. (i) Each modified protein was processed to the mature form but the extent of stop-translocation varied widely. The ratio of the stopped to the translocated products increased as the length and hydrophobicity of the inserted segment increased. (ii) Shorter hydrophobic segments than naturally occurring native transmembrane segment promoted stop-translocation. (iii) Proteins with hydrophobic segments followed by positive charges were more efficiently stop-translocated than those having negative charges. (iv) If the hydrophobicity of the segment was sufficiently high, the positive charges after the segment were not essential for stop-translocation. We also suggest that the stop-transfer process includes protein-protein interaction between the hydrophobic segment and translocation channel.

Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Publishing Authors By Initials

T Kuroiwa,M Sakaguchi,K Mihara,T Omura,

For similar natural sciences: chemistry: chemistry, physical: solubility research abstracts see: natural sciences: chemistry: chemistry, physical: solubility research

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Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 829-34

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1990

Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Keywords Mesh Terms:

KEYWORDS: Solubility

MESH TERMS: metabolism

Chemical & Substance for Abstract: Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane.

AFFILIATION: Department of Biology, Faculty of Science, Kyushu University, Fukuoka.

Country: JAPAN

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MEDLINETA: J Biochem

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