Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47.

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Abstract Text:

Aki Nakaishi,Mayumi Hirose,Masato Yoshimura,Chitose Oneyama,Kazunobu Saito,Nobuharu Kuki,Makoto Matsuda,Nakayuki Honma,Hiroshi Ohnishi,Takashi Matozaki,Masato Okada,Atsushi Nakagawa,Aki Nakaishi,Mayumi Hirose,Masato Yoshimura,Chitose Oneyama,Kazunobu Saito,Nobuharu Kuki,Makoto Matsuda,Nakayuki Honma,Hiroshi Ohnishi,Takashi Matozaki,Masato Okada,Atsushi Nakagawa,Aki Nakaishi,Mayumi Hirose,Masato Yoshimura,Chitose Oneyama,Kazunobu Saito,Nobuharu Kuki,Makoto Matsuda,Nakayuki Honma,Hiroshi Ohnishi,Takashi Matozaki,Masato Okada,Atsushi Nakagawa,

SRC homology 2 domain-containing protein tyrosine phosphatase substrate 1 (SHPS-1 or SIRPalpha/BIT) is an immunoglobulin (Ig) superfamily transmembrane receptor and a member of the signal regulatory protein (SIRP) family involved in cell-cell interaction. SHPS-1 binds to its ligand CD47 to relay an inhibitory signal for cellular responses, whereas SIRPbeta, an activating member of the same family, does not bind to CD47 despite sharing a highly homologous ligand-binding domain with SHPS-1. To address the molecular basis for specific CD47 recognition by SHPS-1, we present the crystal structure of the ligand-binding domain of murine SHPS-1 (mSHPS-1). Folding topology revealed that mSHPS-1 adopts an I2-set Ig fold, but its overall structure resembles IgV domains of antigen receptors, although it has an extended loop structure (C'E loop), which forms a dimer interface in the crystal. Site-directed mutagenesis studies of mSHPS-1 identified critical residues for CD47 binding including sites in the C'E loop and regions corresponding to complementarity-determining regions of antigen receptors. The structural and functional features of mSHPS-1 are consistent with the human SHPS-1 structure except that human SHPS-1 has an additional beta-strand D. These results suggest that the variable complementarity-determining region-like loop structures in the binding surface of SHPS-1 are generally required for ligand recognition in a manner similar to that of antigen receptors, which may explain the diverse ligand-binding specificities of SIRP family receptors.

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Publishing Authors By Initials

A Nakaishi,M Hirose,M Yoshimura,C Oneyama,K Saito,N Kuki,M Matsuda,N Honma,H Ohnishi,T Matozaki,M Okada,A Nakagawa,A Nakaishi,M Hirose,M Yoshimura,C Oneyama,K Saito,N Kuki,M Matsuda,N Honma,H Ohnishi,T Matozaki,M Okada,A Nakagawa,A Nakaishi,M Hirose,M Yoshimura,C Oneyama,K Saito,N Kuki,M Matsuda,N Honma,H Ohnishi,T Matozaki,M Okada,A Nakagawa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of molecular biology

VOLUME: 375

Page Numbers: 650-60

Journal Abbreviation: J. Mol. Biol.

ISSN: 1089-8638

DAY: 7

MONTH: 11

YEAR: 2007

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47.

AFFILIATION: Laboratory of Supramolecular Crystallography, Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Structural Insight into the Specific Interaction between Murine SHPS-1/SIRPalpha and Its Ligand CD47 Related Publications

• 11-Deoxyalisol C and Alisol D: New Protostane-Type Triterpenoids from Alisma plantago-aquatica.
• A case of angioedema associated with decreased C1 inhibitor activity.
• A neural network model of metaphor understanding with dynamic interaction based on a statistical language analysis: targeting a human-like model.
• An adult form of Alexander disease: a novel mutation in glial fibrillary acidic protein.
• Characterization of progenitor domains in the developing mouse thalamus.
• Delay of cataract development in the shumiya cataract rat by the administration of drinking water containing high concentration of magnesium ion.
• Effects of frequency characteristics of reverberation time on listener envelopment.
• Efficacy of living donor liver transplantation for patients with methylmalonic acidemia.
• Efficient generation of antigen-specific cellular immunity by vaccination with poly(gamma-glutamic acid) nanoparticles entrapping endoplasmic reticulum-targeted peptides.
• Fractal-based EEG data analysis of body parts movement imagery tasks.
• Fructose, but not dextrose, accelerates the progression of chronic kidney disease.
• Gamma-aminobutyric acid (GABA) stimulates pancreatic cancer growth through overexpressing GABAA receptor pi subunit.
• Glutathione S-transferase M1 null genotype as a risk factor for carbamazepine-induced mild hepatotoxicity.
• Impact of CYP2D6*10 on H1-antihistamine-induced hypersomnia.
• In Situ Detection of Surface SiH(n) in Synchrotron-Radiation-Induced Chemical Vapor Deposition of a-Si on an SiO(2) Substrate.
• Interfacility transport of the critically ill pediatric patient.
• New Secoiridoid Glucosides from Ligustrum japonicum.
• Nuclear translocation of ADAM-10 contributes to the pathogenesis and progression of human prostate cancer.
• Overexpression of RGPR-p117 suppresses apoptotic cell death and its related gene expression in cloned normal rat kidney proximal tubular epithelial NRK52E cells.
• Pulse radiolysis study on free radical scavenger edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one).
• Rectified momentum transport for a kicked Bose-Einstein condensate.
• TERT promoter-driven adenovirus vector for cancer gene therapy via systemic injection.
• The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons.
• Thiazide diuretics exacerbate fructose-induced metabolic syndrome.
• Uncoupling of the VEGF-endothelial nitric oxide axis in diabetic nephropathy: an explanation for the paradoxical effects of VEGF in renal disease.
• [A patient with small cerebellar infarcts at tonsil and nodulus who complained of vertigo, vomiting and chest oppression]
• [Biological characteristics of an established model of ovarian cancer in mice and its homologous cell lines]
• [Coronary artery aneurysm]
• [Myocardial infarction in young patients]
• [Position of NSAIDs in causal factors of peptic ulcer]