Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs.

Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Research Abstract Details 

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Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Abstract Text:

J Holgersson,P A Jovall,B E Samuelsson,M E Breimer,

Total non-acid glycosphingolipids were isolated from the kidneys of single pigs serologically typed on their red blood cells as blood groups O and A. Glycolipid species were purified by HPLC and structurally characterized by thin-layer chromatography, mass spectrometry, proton NMR spectroscopy, degradation analysis, and reactivity with various monoclonal antibodies, Gal alpha 1-4Gal-specific E. coli bacteria, and lectins. Glucosyl-, globotriaosyl-, and globotetraosylceramides were the predominant molecular species with lactosyl- and globopentaosylceramides (IV3GalGb4Cer) as abundant constituents too. Small amounts of galactosyl- and digalactosylceramides were also present. In the blood group O pig kidneys, blood group H antigens based on four different core saccharides (types 1, 2, 4, and lactosyl core) were identified and the major blood group structure was V2FucIV3Gal-Gb4Cer. In the kidneys from the blood group A pig the corresponding blood group A antigens were found and in addition, a type 3 chain blood group A antigen was indicated by mass spectrometry and by its reactivity with a monoclonal antibody. Trace amounts of the type 2 chain-based X and Y antigens were found while blood group B antigens and the type 1 chain based Lewis antigens could not be detected. The ceramide part of the glycolipids was mainly composed of dihydroxy 18:0 long chain bases and non-hydroxy 16:0-24:0 fatty cids.

Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Publishing Authors By Initials

J Holgersson,PA Jovall,BE Samuelsson,ME Breimer,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 766-77

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1990

Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: immunology

Chemical & Substance for Abstract: Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs. Information

Substance Name: Glycosphingolipids

Registry Number: 0

Grant and Affiliation Information for Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs.

AFFILIATION: Department of Medical Biochemistry, University of Göteborg, Sweden.

Country: JAPAN

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MEDLINETA: J Biochem

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