Structural basis for ligand and heparin binding to neuropilin B domains.

Structural basis for ligand and heparin binding to neuropilin B domains. Research Abstract Details 

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Structural basis for ligand and heparin binding to neuropilin B domains. Abstract Text:

Craig W Vander Kooi,Manuel A Jusino,Benjamin Perman,David B Neau,Henry D Bellamy,Daniel J Leahy,

Neuropilin (Nrp) is a cell surface receptor with essential roles in angiogenesis and axon guidance. Interactions between Nrp and the positively charged C termini of its ligands, VEGF and semaphorin, are mediated by Nrp domains b1 and b2, which share homology to coagulation factor domains. We report here the crystal structure of the tandem b1 and b2 domains of Nrp-1 (N1b1b2) and show that they form a single structural unit. Cocrystallization of N1b1b2 with Tuftsin, a peptide mimic of the VEGF C terminus, reveals the site of interaction with the basic tail of VEGF on the b1 domain. We also show that heparin promotes N1b1b2 dimerization and map the heparin binding site on N1b1b2. These results provide a detailed picture of interactions at the core of the Nrp signaling complex and establish a molecular basis for the synergistic effects of heparin on Nrp-mediated signaling.

Structural basis for ligand and heparin binding to neuropilin B domains. Publishing Authors By Initials

CW Vander Kooi,MA Jusino,B Perman,DB Neau,HD Bellamy,DJ Leahy,

For similar peptides: oligopeptides: tuftsin research abstracts see: peptides: oligopeptides: tuftsin research

PUBMED ID PMID:

MEDLINE DATE:

Structural basis for ligand and heparin binding to neuropilin B domains. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 6152-7

Journal Abbreviation:

ISSN: 0027-8424

DAY: 3

MONTH: 04

YEAR: 2007

Structural basis for ligand and heparin binding to neuropilin B domains. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Structural basis for ligand and heparin binding to neuropilin B domains. Keywords Mesh Terms:

KEYWORDS: Tuftsin

MESH TERMS: chemistry

Chemical & Substance for Abstract: Structural basis for ligand and heparin binding to neuropilin B domains. Information

Substance Name: Tuftsin

Registry Number: 9063-57-4

Grant and Affiliation Information for Structural basis for ligand and heparin binding to neuropilin B domains.

AFFILIATION: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA90466

ACRONYM: CA

MEDLINETA: Proc Natl Acad Sci U S A

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